孕鼠接触葡萄球菌肠毒素B对子代新生鼠TCR Vβ8^+T细胞的影响  

Effect of maternal SEB administration on the TCR Vβ8^+T cells of the offspring newborn rats

作　　者：郑庆委[1] 韦莉[1] 高淑娴[1] 徐志本[1] 周平[1] 徐明珠[2] 陈兰兰[2] 邵棒 申林[3] 管俊昌[1] ZHENG Qing-wei WEI Li GAO Shu-xian XU Zhi-ben ZHOU Ping XU Ming-zhu CHEN Lan-lan SHAO Bang SHEN Lin GUAN Jun-chang(Anhui Key Laboratory of Infection and Immunity Department of Biological Sciences Scientific Research Center ,Bengbu Medical College ,Bengbu Anhui 233030, China)

机构地区：[1]蚌埠医学院感染与免疫重点实验室,安徽蚌埠233030 [2]蚌埠医学院生物科学系,安徽蚌埠233030 [3]蚌埠医学院科研中心,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2017年第3期281-284,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金(81571454);安徽省高校自然科学研究项目重点项目(KJ2015A211);安徽省高校自然科学研究项目一般项目(KJ2015B081by);安徽省重点实验室(蚌埠医学院)开放课题(BYKL1045ZD);国家级大学生创新训练项目(201510367013)

摘　　要：目的:观察孕鼠接触葡萄球菌肠毒素B(SEB)对其子代新生鼠胸腺及外周血TCR Vβ8^+T细胞的影响。方法:在妊娠16 d时给予SD大鼠尾静脉注射15μg SEB(SEB组),同时设立注射磷酸盐缓冲溶液对照组。孕鼠自然分娩后获取出生后0~5 d的子代新生鼠胸腺及外周血,流式细胞仪检测其TCR Vβ8^+T细胞比例。结果:与对照组比较,SEB组出生后0~3 d的新生鼠胸腺中CD4^+Vβ8^+T细胞比例均减少(P<0.05~P<0.01),出生后4~5 d 2组差异均无统计学意义(P>0.05);出生后0~5 d,SEB组胸腺中CD8^+Vβ8^+T细胞比例均低于对照组(P<0.05~P<0.01);出生后0~5 d,SEB组CD4^+Vβ8^+及CD8^+Vβ8^+T细胞的绝对数均较对照组明显减少(P<0.01)。新生鼠出生后0~5 d,SEB组外周血CD4^+Vβ8^+T细胞比例均较对照组明显减少(P<0.01);CD8^+Vβ8^+T细胞比例亦均较对照组减少(P<0.05~P<0.01);出生后0~5 d,SEB组外周血CD4^+Vβ8^+及CD8^+Vβ8^+T细胞绝对数均较对照组明显减少(P<0.01)。结论:妊娠期大鼠接触SEB可减少子代新生鼠胸腺及外周血TCR Vβ8^+T细胞,并保留至成年期。Objective：To investigate the effects of maternal SEB administration on the TCR Vβ8^＋T cells in the thymus and peripheral blood of offspring newborn rats. Methods：Pregnant rats with gestational 16 days were injected with 15 p,g SEB in 0.2 mL PBS by iv （ SEB group） , and the control group were treated with the same volume of PBS. The thymus and peripheral blood of newborn offspring rats after 0 to 5 days of delivery were harvested, and the levels of TCR Vβ8^＋ T cells of whose were analyzed using the flow cytometry. Results：Compared with the control group,the percentage of CD4^＋ TCR Vβ8^＋T cells in thymus of offspring newborn rats after 0 to 3 days of delivery significantly decreased in SEB group （ P 〈 0.05 to P 〈 0.01 ）, and the difference of which in thymus of offspring newborn rats after 4 to 5 days of delivery between two groups was not statistically significnat （ P 〉 0.05 ）. In the thymus of offspring newborn rats after 0 to 5 days of delivery, the percentage of CD8^＋ TCR Vβ8^＋ T ceils in SEB group was significantly lower than that in control group（P 〈 0.05 to P 〈 0.01 ）, and the absolute number of CD4^＋Vβ8^＋ and CD8^＋ Vβ8^＋ T ceils in SEB group significantly decreased compared with the control group（ P 〈 0.01 ）. In the peripheral blood of offspring newborn rats after 0 to 5 days of delivery ,the percentages of the CD4^＋ Vβ8^＋ and CD8^＋ Vβ8^＋ T cells, and the absolute number of CD4^＋ Vβ8^＋ and CD8^＋ Vβ8^＋ T cells in SEB group significantly decreased compared with the control group（ P 〈 0.05 to P 〈 0.01 ）. Conclusions： Maternal SEB administration during pregnancy can decrease the levels of TCR Vβ8^＋ T cells in the thymus and peripheral blood of offspring newborn rats ,and which can continue into adulthood.

关 键 词：妊娠并发症 葡萄球菌肠毒素B TCR Vβ8^+T细胞 大鼠 

分 类 号：R714.25[医药卫生—妇产科学]