乌司他丁干预对脓毒症幼鼠凝血功能的影响  

Impact of ulinastatin on the Blood coagulation function in sepsis rat

作　　者：刘赟[1] 程鹏[1] LIU Yun CHENG Peng.(Department of Pediatrics ,First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

机构地区：[1]南昌大学第一附属医院儿科,南昌330006

出　　处：《江西医药》2017年第5期400-403,共4页Jiangxi Medical Journal

摘　　要：目的探讨使用乌司他丁(UTI)作为早期干预药物对脓毒症凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血小板(PLT)的影响及意义。方法选用4周龄健康清洁级雄性大鼠120只,改良的CLP方法制作脓毒症大鼠模型,随机分5组:正常对照组、假手术组、脓毒症组、低剂量UTI干预组(50000U/kg)、乌司高剂量他丁干预组(200000U/kg),每组24只鼠。测定6h、12h和24h PT、APTT、PLT,行统计学分析。结果在6h时间段各组实验幼鼠PT、APTT、PLT水平无明显差异,12h时点,脓毒症组PT、APTT出现明显上升,而血小板出现下降(P<0.05),24h时点PT值进行性上升,但APTT值呈下降趋势,而PLT却呈显著下降,低剂量UTI干预组PT、APTT、PLT水平在12h、24h均有不同程度的上升,变化规律与脓毒症组相同,但低于脓毒症组同时点PT、APTT水平(P<0.05),PLT值却高于脓毒症组同时间点PLT水平,高剂量UTI干预组PT、APTT、PLT水平变化趋势与低剂量UTI干预组相同,PT、APTT值在12h、24h均较低剂量UTI干预组降低(P<0.05),PLT值在12h、24h均较低剂量UTI干预组增高(P<0.05),具有显著差异,两组相比较,高剂量组较低剂量组作用更显著。结论 UTI早期干预对脓毒症幼鼠模型的PT、APTT、PLT均有改善,且高剂量组较低剂量组作用更显著。Objective To investigate the influence and significance of ulinastatin as an early intervention drug on PT,AprIT and PLT of sepsis rat. Methods 120 four-week-old healthy clean grade male rats were selected and divided randomly and evenly into 5 groups：blank control group, sham-operation group, sepsis group （using modified cecal ligation puncture （CLP） method）, ulinastatin intervention （50000U/kg and 200000U/kg） groups. The blood of each group was analysed at different time interval （6h, 12h and 24h） for PT, APTT and PLT measurement. Results There was no significant difference between all groups for PT, APT and PLT when measured in 6 hour interval ;for sepsis group ,when measured in 12 hour interval ,PT and APTF significantly increased, PLT decreased （P〈0.05）,when measured in 24 hour interval,PT continued increased,but APTT showed a decreasing trend,PLT decreased even more ;for 50000U/kg ulinastatin intelvention group ,PT, APTT and PLT all increased both in 12 hour interval and 24 hour interval. Increasing trend was similar to corresponding increasing trend shown in sepsis groupz,but PT and APTT were quantitatively lower than sepsis group（P〈0.05）, PLT was quantitatively higher than sepsis group. Analysis of PT, APTT and PLT for 200000U/kg ulinastatin intervention really works much better than 50000U/kg ulinastatin intervention. Conclusion Early UTI intervention do help in PT,APTT and PLT fpr sepsis rat,and higher dose has more remarkable effect.

关 键 词：UTI 脓毒症 PT APTT 血小板 大鼠 

分 类 号：R631[医药卫生—外科学]