紫外线吸收剂PBSA与DNA相互作用的光谱研究  

Study on the interaction of ultraviolet absorbent PBSA with DNA by spectroscopic method

作　　者：陈砚美[1] 杨晗[1] 王芳[1] 熊绪杰[1] 张万举[1] CHEN Yanmei YANG Han WANG Fang XIONG Xujie ZHANG Wanju(Hubei Key Laboratory for Processing and Application of Catalytic Materials, Huanggang Normal University, Huanggang, Hubei 43800)

机构地区：[1]黄冈师范学院催化材料制备与应用湖北省重点实验室,湖北黄冈438000

出　　处：《华中师范大学学报（自然科学版）》2017年第3期324-327,共4页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省自然科学基金项目(2016CFB147)

摘　　要：采用荧光光谱法和紫外光谱法,在模拟生理条件下(pH=7.23)对紫外线吸收剂2-苯基苯并咪唑-5-磺酸(PBSA)与脱氧核糖核酸(DNA)的相互作用进行了研究.结果表明PBSA能够与DNA相结合形成复合物,引起了PBSA紫外吸收的降低和荧光的猝灭;PBSA与DNA的相互作用为静态猝灭过程,两者间的结合位点数为1.11.The interaction of ultraviolet absorbent 2-phenyl benzimidazol-5-sulfonic acid （PBSA） and DNA in physiological condition was investigated by fluorescence spectrometry and UV absorption spectrophotometry. The results showed that PBSA interacted with DNA in an electrostatic interaction mode, which resulted in the hypochromic effect as well as fluorescence quenching of PBSA. The combination reaction of them was a sin- gle static quenching process, and the binding site was 1.11.

关 键 词：2-苯基苯并咪唑-5-磺酸(PBSA) DNA相互作用 光谱法 

分 类 号：O626.2[理学—有机化学]