海南南繁基地玉米茎腐病病原菌的分离及鉴定  

The isolation and identification of maize stalk rot pathogen in Hainan Southern Fan Base

作　　者：牛艺诺 林宝英 刘全凤[3] 刘永震 吴金山[1] 戎伟[1] 梅双双[1] NIU Yinuo LIN Baoying LIU Quanfeng LIU Yongzhen WU Jinshan RONG Wei MEI Shuangshuang(College of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737 Hainan Institute for Food Control, Haikou 571001 Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou, Hebei 061001 Cangzhou Plant Protection and Quarantine Station, Cangzhou, Hebei 061000)

机构地区：[1]海南大学热带农林学院,海南儋州571737 [2]海南省食品检验检测中心,海口571001 [3]沧州市农林科学院,河北沧州061001 [4]沧州市植物保护检疫站,河北沧州061000

出　　处：《华中师范大学学报（自然科学版）》2017年第3期345-348,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(31560296);海南省自然科学基金项目(314057;314057)

摘　　要：为明确海南地区南繁基地玉米茎腐病的病原,从玉米发病组织中分离病原菌,并进行了纯化培养、革兰氏染色、病原菌回接等试验,得到了2株对玉米有较强致病力的菌株,分别命名为JF02与JF04.随后通过PCR反应扩增了所分离的玉米茎腐病病原菌JF02与JF04的16SrRNA序列,并测序,序列分析表明,所分离的玉米茎腐病病原菌序列相同,属同一病原菌,且为狄克氏菌.该病原菌的分离和鉴定为玉米细菌性茎腐病的防治和抗性品种选育提供了一定的理论参考.To identify the pathogen of maize bacterial stalk rot disease occurred in Southern Fan Base of Hainan Province, the pathogenic bacteria JF02 and JF04 were isolated from the maize stalk rot tissue. Back inoculation assay confirmed that JF02 and JF04 are the pathogen of maize stalk rot disease. In addition, the bacteria strain JF02 and JF04 display the same 16S rRNA sequence and is very closed to Dickeya zeae EC1 （99 %）, which indicated that JF02 and JF04 are the same and belongs to Dickeja zeae. Overall, this study provide references for the control of maize bacterial stalk rot disease and future screening of resistant maize inbred line.

关 键 词：玉米茎腐病 病原菌 狄克氏菌 

分 类 号：Q94[生物学—植物学]