夏枯草幼苗中有效成分与生理指标对增强UV-B辐射的动态响应  

The dynamic response of enhanced UV-B radiation on Prunella vulgaris L. seedlings active ingredients and physiological indexes

作　　者：王园[1] 党悦方[1] 张典[1] 赵阿静 陈千良[1] 房敏峰[1] WANG Yuan DANG Yuefang ZHANG Dian ZHAO Ajing CHEN Qianliang FANG Minfeng(Key Laboratory of Resource Biological and Biotechnology in Western China, College of Life Sciences, Northwest University, Xi＇an 710069, China)

机构地区：[1]西北大学生命科学学院/西部资源生物与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2017年第3期414-421,共8页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(J1210063);西北大学科研启动基金资助项目(20160416)

摘　　要：以药用植物夏枯草幼苗为研究对象,采用低(CK)、中(UV-B+)、高(UV-B++)不同强度UV-B辐射对材料进行处理,设置自然光照为对照,于处理后0~60 d进行测定,探究增强UV-B辐射对夏枯草幼苗不同发育期9种主要有效成分及有关生理指标的影响。分析结果显示,类胡萝卜素在不同强度UV-B辐射下含量有显著增加,处理后期(≥40 d)增强UV-B辐射使花青素含量增加,不同强度UV-B辐射下的抗氧化酶活力均有显著增加。UV-B辐射可促进夏枯草中金丝桃苷含量积累、其他有机酸及黄酮类各成分在一定时间内对UV-B辐射有含量增加的响应,低强度UV-B辐射于处理后期(≥50 d)可显著提升三萜类成分的含量。由此可见,夏枯草幼苗对外界UV-B辐射增强可产生适应与防御机制,适度增强UV-B辐射可促进药用成分的积累。Using the Prunella vulgaris L. seedlings as the research object, with CK, UV-B ＋ , UV-B ＋ ＋ degree of different UV-B radiation on materials for processing, set the natural light to contrast, determined after treatment in 0 - 60 d, explore the effects of enhanced UV-B radiation on the Prunella vulgaris L. seedlings of 9 kinds of the influenced the main effective components and related physiological indexes. The analysis results showed that the carotenoids content under different intensity of UV-B radiation has increased significantly, enhanced UV-B radiation in late processing （ ≥40 d） make anthocyanin content increases, antioxidant enzymes activity under different intensity of UV-B radiation were increased; UV-B radiation can promote accumulation of hyperoside content in Prunella vulgaris L. , other phenolic acids and flavonoids of each component in a certain time of UV-B radiation levels had increased response, low intensity UV-B radiation in the late processing （ ≥50 d） can significantly improve the content of triterpene composition. Thus, Prunella vulgaris L seedlings can produce the adaptive and defense mechanism in enhanced UV-B radiation, moderately enhanced UV B radiation can promote the accumulation of medicinal ingredients.

关 键 词：夏枯草 UV-B辐射 有效成分 生理指标 动态响应 

分 类 号：Q945.79[生物学—植物学]