LRP16肝特异性基因敲除小鼠模型的构建与鉴定  

Construction and identification of liver-specific leukemia-related protein 16 gene knockout mice model

作　　者：安平[1] 王安平[1] 孟秋涛 母义明[1] AN Ping WANG Anping MENG Qiutao MU Yiming(Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China)

机构地区：[1]解放军总医院内分泌科,北京100853

出　　处：《解放军医学院学报》2017年第7期649-652,674,共5页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81471026)~~

摘　　要：目的建立白血病相关蛋白16(leukemia-related protein 16,LRP16)肝脏特异性基因敲除小鼠模型,进而在体内研究LRP16基因与胰岛素抵抗的关系。方法构建LRP16基因重组载体,通过电转打靶转染胚胎干细胞(ES细胞),将筛选出的阳性ES细胞克隆,显微注射至超排小鼠的囊胚腔后移植于受体小鼠,从子代中筛选嵌合体小鼠,与野生型C57小鼠杂交后得到LRP16-Loxp转基因小鼠,再与Alb-Cre小鼠杂交后得到LRP16肝特异性基因敲除小鼠并进行鉴定。结果得到8株阳性ES细胞克隆,经显微注射后得到2只雄性嵌合体小鼠,与野生小鼠杂交后得到1只LRP16-Loxp转基因小鼠,与Alb-Cre小鼠杂交后再自交筛选出2只小鼠经鉴定为LRP16肝特异性基因敲除小鼠。结论成功建立LRP16肝特异性基因敲除小鼠模型,为研究LRP16基因与胰岛素抵抗的关系奠定基础。Objective To establish liver-specific leukemia-related protein 16 （LRP16） gene knockout mice model, and investigate the relationship between LRP16 gene and insulin resistance. Methods The knockout vector targeting exon 5 to exon 8 of LRP16 gene was constructed by molecular cloning and transfected to embryonic stem （ES） cell by electroporation. The selected positive ES cells was microinjected into the blastula of C57BL/6J mice. And the chimerical mice were born after transplantation of blastula into host mice. The LRP16-Loxp mice were born by crossing between chimerical mice and C57BL/6J mice, and the liver-specific LRP16 knockout mice were obtained by crossing between LRP16-Loxp mice and Alb-Cre mice. Results Eight positive ES cell cloning strains were obtained, and 2 chimerical mice, 1 LRP16-Loxp mouse and 2 liver-specific LRP16 knockout mice were obtained to meet the research demand. Conclusion Liver-specific LRP16 knockout mice model is successfully established, which will be helpful in studying the correlation between LRP16 and insulin resistance.

关 键 词：白血病相关蛋白16 条件性基因敲除 LRP16 基因敲除小鼠模型 

分 类 号：R965[医药卫生—药理学]