颅内隐球菌感染对脑屏障通透性的影响  

Influence of intracranial cryptococcal infection on brain barrier permeability

作　　者：郑娜[1] 王威[1] 张家堂[1] 邵龙[1] 蒋胶胶 ZHENG Na WANG Wei ZHANG Jiatang SHAO Long JIANG Jiaojiao(Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China)

机构地区：[1]解放军总医院神经内科,北京100853

出　　处：《解放军医学院学报》2017年第7期668-670,共3页Academic Journal of Chinese PLA Medical School

摘　　要：目的探索颅内隐球菌感染对脑屏障通透性的影响。方法成年雄性SD大鼠12只,体质量260~300 g,随机分为对照组和实验组,各6只。实验组大鼠建立隐球菌脑膜炎模型,对照组为正常大鼠。尾静脉给予氟康唑,利用纹状体、侧脑室同步微透析联合高效液相色谱法检测血浆、脑细胞外液和脑脊液中氟康唑浓度。计算氟康唑在血-脑屏障和血-脑脊液屏障的透过率,观察颅内隐球菌感染对氟康唑血-脑屏障、血-脑脊液屏障的透过率的影响。结果正常组大鼠血-脑屏障及血-脑脊液屏障透过率分别为62.12%±10.38%和69.33%±7.34%;实验组大鼠血-脑屏障及血-脑脊液屏障透过率分别为78.18%±2.45%和87.29%±7.11%,实验组血-脑屏障和血-脑脊液屏障透过率均比对照组高(P均<0.05)。结论颅内隐球菌感染增加了血-脑屏障和血-脑脊液屏障的通透性。Objective To investigate the influence of intracranial cryptococcal infection on brain barrier permeability. Methods Twelve adult male SD rats weighting 260-300 g were randomly divided into control group and experimental group （n=6 in each group）. Cryptococcal meningitis rat model was established in experimental group. Fluconazole was administered via the tail vein and micro dialysis sampling was used to obtain extracellular fluid and cerebrospinal fluid synchronously. The concentrations of fluconazole in plasma, extracellular fluid and cerebrospinal fluid were measured using high performance liquid chromatography （HPLC）. The permeation rate of fluconazole in blood brain barrier and blood cerebrospinal fluid barrier in different groups were calculated to investigate the influence of intracranial cryptococcal infection on brain barrier permeability. Results The permeation rates of fluconazole through blood - brain barrier and blood - cerebrospinal fluid barrier were 62.12%±10.38% and 69.33%±7.34% respectively in the normal group, significantly higher than those of 78.18%±2.45% and 87.29%±7.11% in the experimental group （P ＜ 0.05, respectively）. Conclusion Intracranial cryptococcal infection can increase the permeability of blood - brain barrier and blood - brain fluid barrier.

关 键 词：氟康唑 血- 脑屏障 血- 脑脊液屏障 隐球菌脑膜炎 

分 类 号：R379.5[医药卫生—病原生物学]