加味复肾汤对DN大鼠肾脏病变的影响  

The Effects of Jiawei Fushen Tang on Renal Lesion in Diabetic Nephropathy Rats

作　　者：陈雯琳 刘华东[2] 李璇[3] 张卫华[1] 艾迁明 罗斌[1] 冯鸣[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210023 [2]南京中医药大学第二临床医学院,江苏南京210023 [3]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2017年第3期273-278,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省教育厅科研项目(12KJB360003);江苏省优势学科建设工程项目(PAPD)

摘　　要：目的观察加味复肾汤(JWFST)对DN大鼠肾脏病变的影响,并探讨其可能的作用机制。方法雄性Wistar大鼠40只,分为5组,正常组8只,其余大鼠腹腔注射STZ+CFA建立糖尿病模型。造模成功后分为模型组(M)、JWFST小剂量组(L1)、JWFST中剂量组(L2)、JWFST大剂量组(L3)。L1、L2、L3组分别按8、16、32g/(kg·d)剂量灌胃;模型组以生理盐水灌胃。连续8周,每周定期测血糖。8周后,收集24h尿液,采集血清,检测INS、SCr、BUN、SOD、MDA、UP等生化指标;光镜下观察肾脏组织病理变化;免疫荧光法检测肾组织nephrin和podocin的表达,qPCR法检测肾组织nephrin、podocin的基因表达。结果与模型组比较,L1、L2、L3组的SCr、BUN、MDA、UP明显降低(P<0.05~0.01),SOD明显升高(P<0.05),病理学评分明显降低(P<0.05),nephrin、podocin的免疫荧光表达明显上调(P<0.01),qPCR结果表明,nephrin、podocin基因表达明显上调(P<0.05)。结论 JWFST可以有效改善DN大鼠的肾脏损害,对糖尿病肾病病情的发展具有一定的延缓作用。OBJECTIVE To observe the effects of Jiawei Fushen Tang on diabetic nephropathy rats with renal lesions and discuss the possible mechanism. METHODS 40 male SD rats were treated with intraperitoneal injection of STZ＋CFA to es- tablish diabetic model after normal feeding for 1 weeks. After successful modeling, the rats were divided into model group （M）, JWFST （L1） group,JWFST （L2） group,JWFST（L3） group. L1, L2, L3 group were treated with JWFST 8 g/（kg.d）, 16 g/（kg.d）, 32 g/（kg.d） ; the model group was given normal saline; the normal control group （C） was 8 male SD rats with conventional feeding. The blood sugar on a regular basis for 8 weeks were recorded. After the experiment, collect 24 h urine, the serum was collected and biochemical parameters, like SCr, BUN, SOD, MDA, UPwere detected. Then the kidneys were harvested and the expressions of nephrin and podoein in the kidney were detected by IF method and qPCR method. And then observed the renal lesions under light microscope. RESULTS Compared with the model group, the INS, SCr, BUN, MDA, UP of treatment groups were significantly decreased, and SOD was increased significantly. The expressions of nephrin and podocin were raised significantly by JiaWeiFuShenTang. CONCLUSION JiaWeiFuShenTang can significantly improve the re- nal damage of DN rats, and delay the development of diabetic nephropathy.

关 键 词：加味复肾汤 糖尿病肾病 DN大鼠 NEPHRIN PODOCIN 

分 类 号：R285.5[医药卫生—中药学]