巴戟天与雌激素对骨质疏松大鼠破骨细胞RANK和CAⅡ的表达影响  

Effect of morinda officinalis and estrogen on RANK and CAⅡ expression in osteoclasts of osteoporosis rat

作　　者：杨博辰[1] 朱明喜[1] 曹一维 

机构地区：[1]河南中医药大学第三附属医院骨科,郑州450000

出　　处：《天津医科大学学报》2017年第3期203-207,共5页Journal of Tianjin Medical University

摘　　要：目的:探讨巴戟天、雌激素对骨质疏松大鼠破骨细胞核激活因子κβ受体(RANK)和碳酸酐酶Ⅱ(CAⅡ)的表达水平间关系。方法:选择月龄为4个月的SPF级成年健康雌性SD大鼠,建立去势大鼠骨质疏松模型,分离大鼠原代破骨细胞并分为6组:空白对照组、17-雌二醇10^(-6) mmol/L组、0.1 g/d巴戟天组、0.5 g/d巴戟天组、1.0 g/d巴戟天组、17-雌二醇10^(-6) mmol/L联合1.0 g/d巴戟天组进行培养,观察比较各组CAⅡ、RANK mRNA表达等指标差异。结果:17-雌二醇10^(-6) mmol/L组、0.5 g/d巴戟天组、1.0 g/d巴戟天组、17-雌二醇10^(-6) mmol/L联合1.0 g/d巴戟天组破骨细胞数量均较空白对照组明显减少,而17-雌二醇10^(-6)mmol/L联合1.0 g/d巴戟天组减少最明显(P<0.05),0.1 g/d巴戟天组与对照组无显著差异(P>0.05);17-雌二醇10^(-6) mmol/L组、0.5 g/d巴戟天组、1.0 g/d巴戟天组、17-雌二醇10^(-6) mmol/L联合1.0 g/d巴戟天组骨吸收陷窝面积相均低于空白对照组(P<0.05),而17-雌二醇10^(-6) mmol/L联合1.0 g/d巴戟天组骨吸收陷窝面积明显低于其他组(P<0.05),0.1 g/d巴戟天组与对照组无显著差异(P>0.05);17-雌二醇10^(-6) mmol/L组、0.5 g/d巴戟天组、1.0 g/d巴戟天组、17-雌二醇10^(-6) mmol/L联合1.0 g/d巴戟天组CAⅡ、RANK mRNA基因表达水平明显低于空白对照组(P<0.05),0.1 g/d巴戟天组与对照组无显著差异(P>0.05),17-雌二醇10^(-6) mmol/L组和0.5 g/d巴戟天组RANK mRNA基因、CAⅡ mRNA基因表达水平差异无统计学意义(P>0.05),17-雌二醇10^(-6)mmol/L联合1.0 g/d巴戟天组CAⅡ、RANK mRNA基因表达水平最低(P<0.05)。结论:巴戟天联合雌激素能有效诱导骨质疏松大鼠破骨细胞内RANK和CAⅡ处于低表达状态,从而有效预防骨质疏松的发生。Objective： To investigate the function of morinda officinalis and estrogen on RANK and CAⅡ expression in osteoclasts of osteoporosis rat. Methods：Four-months old SFP healthy female rats were chosen for osteoporosis rat model construction. Primary osteoclast cells were isolated and divided into 6 groups, then they were treated with control, 10-6mmol/L 17-estradiol, 0.5 g/d morinda officinalis,1.0 g/d morinda officinalis, 10-6 mmol/L 17-estradiol combined with1.0 g/d morinda officinalis respectively. Then osteoclast number, the area of bone resorption, the mRNA expression of carbonic anhydrase Ⅱ and beta receptor activator of nuclear factor were calculated. Results：Osteoclast numbers for 10-6mmol/L 17-estradiol, 0.5 g/d morinda officinalis, 1.0 g/d morinda officinalis, and 10-6mmol/L 17-estradiol combine with 1.0 g/d morinda officinalis were less than the control（P〈0.05）. The group of 0.1 g/d morinda officinalis had no significance with the control（P〉0.05）. The group of 10-6 mmol/L 17-estradiol combined with 1.0 g/d morinda officinalis was the least（P〈0.05）. The expressions of carbonic anhydrase Ⅱ and beta receptor activator of nuclear factor in 10-6 mmol/L 17-estradiol, 0.5 g/d morinda officinalis, 1.0 g/d morinda officinalis,10-6mmol/L 17-estradiol combine with 1.0 g/d morinda officinalis groups were lower than the control（P〈0.05）. The group of 0.1 g/d morinda officinalis had no significance compared with the control（P〉0.05）. The group of 10-6mmol/L17β-estradiol combined with 1.0 g/d morinda officinalis had the lowest expression（P〈0.05）. But the expressions of carbonic anhydrase Ⅱ and beta receptor activator of nuclear factor between 10-6mmol/L 17-estradiol group and 0.5 g/d morinda officinalis group had no significant difference（P〉0.05）. Conclusion：Estradiol combined with morinda officinalis could decrease the expression of RNAK and CAⅡ to prevent osteoporosis in osteoporosis rat.

关 键 词：巴戟天 雌激素 骨质疏松 破骨细胞 核激活因子κβ受体 碳酸酐酶II 大鼠 

分 类 号：R589.5[医药卫生—内分泌]