ATP生物荧光法评价医疗器械清洗效果观察  

Observation on the ATP bioluminescence assay used for evaluation of cleaning quality of medical instruments

作　　者：何学军[1] 蒋国钦[1] HE Xue -jun JIANG Guo -qin(The Center for Disease Control and Prevention of Shaoxing,Shaoxing Zhejiang 312071 ,China)

机构地区：[1]绍兴市疾病预防控制中心,浙江绍兴312071

出　　处：《中国消毒学杂志》2017年第5期428-430,共3页Chinese Journal of Disinfection

摘　　要：目的观察ATP生物荧光法评价医疗器械清洗效果及影响因素。方法采用ATP生物荧光法,评价医疗器械清洗效果,同时与目测法作比较。结果按照本研究清洗程序清洗后的240件医疗器械肉眼裸视法检测合格率98.8%,放大镜观察法检测合格率82.1%;ATP生物荧光法检测结果显示,不同清洗方法、不同存放时间的医疗器械清洗后所测定的RLU对数值之间有显著统计学意义(P≤0.001)。多元线性回归分析发现,ATP荧光法检测值(RLU)的对数值与清洗方法、存放时间呈正线性相关(P<0.001),回归模型显著(F=22.38,P<0.001),但校正R2为0.20,拟合优度一般。结论该医院医疗器械肉眼裸视法及放大镜观察法合格率较高,清洗方法、存放时间可影响医疗器械清洗效果,ATP荧光检测法检测结果与肉眼观察法差异较大。Objective To evaluate the cleaning efficacy for medical instrument by ATP bioluminescence assay and to find the influence factors of ATP value by multiple regressions. Methods Cleaning efficacy for medical instrument was evalua- ted by ATP bioluminescence assay and the result was compared with that of naked eye observation. Results The qualified rate of the medical instrument was 98.8% by naked eye observation, which was the higher than that by magnifying glass ob- servation 82.1%. Single factor analysis for the exp（ ATP value） found that different cleaning methods and store time had significant different exp（ ATP value） ,P ≤0. 001, statistically. Multiple linear regression found the relationship between exp （ ATP value） and cleaning methods and store time was linear and positive（ P 〈 0. 001 ）. The linear regression model was sig- niflcant（F =22.38 ,P 〈0. 001 ） ,but the adjusted R2 was 0.20 and the goodness of fit was bad. Conclusion The qualified rates of the medical instrument are high by naked eye observation and magnifying glass observation. Cleaning methods and store time may influence the quality of cleaning for medical instruments. The result between ATP biol naked eye observation is different significantly. assay and

关 键 词：ATP生物荧光法 肉眼裸视法 清洗效果 影响因素 

分 类 号：R187[医药卫生—流行病学]