13种中草药对禽源葡萄球菌体外抑菌试验  

Antibacterial Effect of Thirteen Kinds of Traditional Chinese Herbs against Staphylococcus Isolates from Poultry in Vitro

作　　者：丁月霞[1] 黎梓葱 张秋玲[1] 林勇志[1] 林鸿娟 林杰仪 

机构地区：[1]广东海洋大学农学院,广东湛江524088

出　　处：《畜牧与饲料科学》2017年第4期68-70,共3页Animal Husbandry and Feed Science

基　　金：广东海洋大学大学生创新创业训练计划项目资助(524000087205)

摘　　要：采用试管二倍稀释法分别测定13种中草药对临床分离的禽源葡萄球菌的最小抑菌浓度(minimal inhibitory concentration,MIC),筛选出抑菌作用较好且临床常用的5味中草药,并采用棋盘稀释法测定中草药联用后对禽源葡萄球菌的微量抑制浓度指数(fractional inhibitory concentration index,FIC index),旨在研究中草药联用对禽源葡萄球菌的体外抑菌效果。结果表明,13味中草药水提物中,钩藤、乌梅对禽源葡萄球菌的体外抑菌作用最强,MIC均小于1.95 mg/m L;其次为黄连和苏木,MIC为3.91 mg/m L。在联用试验中,忍冬与诃子联用表现为协同作用,其他组合表现均为无关或颉颃作用。The aim of the present study was to evaluate the antibacterial effect of single and combined application of traditional Chinese herbs against Staphylococcus isolates from poultry in vitro. The minimal inhibitory concentration （MIC） of thirteen kinds of traditional Chinese herbs against clinical isolates of poultry origin Staphylococci was determined by tube double dilution method. Five kinds of traditional Chinese herbs with better antibacterial activity were screened, and the fractional inhibitory concentration index （FIC index ） against Staphylococci when the five kinds of traditional Chinese herbs were combinedly used was determined by checkerboard dilution method. The results showed that the aqueous extract of uncaria and dark plum showed the best antibacterial activity in vitro against the poultry origin Staphylococci and both of their MIC was less than 1.95 mg/mL, followed by that of coptis and sappan wood with MIC of 3.91 mg/mL. The synergistic antibacterial effect was observed in the combined application of honeysuckle and myrobalan, and the other combinations exhibited irrelevant or antagonistic action.

关 键 词：中草药 葡萄球菌 最低抑菌浓度 

分 类 号：S853.75[农业科学—临床兽医学]