高渗胁迫对玉米大斑病菌生长发育的影响及菌丝细胞中渗透调节物质的分析  

Effect of Hyperosmotic Stress on Growth and Development of Setosphaeria turcica and Determination of Osmolytes in the Mycelium Cells of the Pathogen

作　　者：巩校东[1] 刘星晨[1] 赵立卿 郑亚男[1] 范永山[2] 韩建民[1] 谷守芹[1] 董金皋[1] 

机构地区：[1]河北农业大学真菌毒素与植物分子病理学实验室,河北保定071001 [2]唐山师范学院生命科学系,河北唐山063000

出　　处：《中国农业科学》2017年第10期1922-1929,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31171805;31271997;31371897);河北省自然科学基金(C2014105067;C2016204164);河北省研究生创新资助项目(1099009)

摘　　要：【目的】了解不同高渗胁迫条件对玉米大斑病菌(Setosphaeria turcica)菌丝发育及胞内黑色素含量的影响,明确在病菌菌丝细胞中起主要作用的渗透调节物质的种类及这些物质在不同高渗胁迫条件下的变化规律。【方法】采用3种不同浓度梯度(0.4、0.8、1.2 mol·L-1 Na Cl)的高渗胁迫条件处理玉米大斑病菌,分析高渗胁迫对菌落生长、菌丝发育及菌丝胞内黑色素含量的影响;利用高效液相色谱(high performance liquid chromatography,HPLC)技术测定3种高渗浓度处理下菌丝细胞中甘油、赤藓醇、葡萄糖、甘露醇、海藻糖5种多羟基醇的含量并分析其随时间变化的规律。【结果】与在普通PDA培养基上的病菌相比,在高渗胁迫条件下病菌菌落生长速率明显降低,菌丝细胞间隔变短、细胞显著膨大,且随胁迫浓度增加细胞膨大程度增强;高渗胁迫下的菌株在各个时间点的胞内黑色素含量与对照相比有明显差异,其中高渗处理12 h之前样品中黑色素的含量均比对照组低,但不同处理之间含量均很接近;而在24、48 h两个时间点不同处理浓度对黑色素的影响不同,1.2 mol·L-1Na Cl处理下与对照相比黑色素含量均显著增加,0.8 mol·L-1 Na Cl处理下与对照相比黑色素含量降低,0.4 mol·L-1Na Cl处理下样品的黑色素含量与对照相比在24 h时显著降低,但48 h时与对照基本一致;在不同的高渗胁迫条件下(0.4、0.8、1.2 mol·L-1 Na Cl)菌丝细胞中甘露醇的含量均有随处理时间延长而增加的趋势,在处理时间为36 h时甘露醇含量较对照增加最为明显,具有显著性差异;菌丝中甘油含量的变化与甘露醇的变化规律相似,且在高渗胁迫处理下甘油含量增加幅度更加明显,并在处理24 h后甘油含量较对照显著增加;菌丝中海藻糖含量总体上有随时间延长而降低的趋势,且渗透胁迫的强度越高该趋势越明显,不同浓度Na Cl处理的菌丝中海藻糖�[Objective] The objective of this study is to understand the effect of hyperosmotic stress on the growth and development, melanin content in mycelium cells, determine the probable osmolytes in the mycelium cells and to clarify the variation rule of these substances under different hyperosmotic stress conditions in Setosphaeria turcica. [Method] The effects of hyperosmotic stress on colony growth rate, mycelium morphological characteristics and melanin content in S. turcica mycelium cell were analyzed under different hyperosmotic stress media, in which 0.4, 0.8, and 1.2 mol＇L-1 NaC1 were added in PDA medium. High performance liquid chromatography （HPLC） technology was employed to detect the content of polyhydroxy-alcohol including glycerol, erythrol, glucose, mannitol, trehalose and analyzed the change profiles of these substances as time increased. [Result] Compared to the strain cultured on PDA medium, the strain cultured under hyperosmotic stress treatments appeared with decreased in colony growth rate, shortened septum and swollen cells. Melanin content in mycelium cells cultured under hyperosmotic stress treatments in all time points showed a significant difference compared to the control group, in which melanin content in the samples treated before 12 h were lower than that in control, but there were no obvious differences between the samples treated. Moreover, there were different effects under different times （24, 48 h） and different concentration treatments. Compared to the control group, melanin content in the samples treated with 1.2 mol·L-1 NaC1 was significantly increased, melanin content treated with 0.8 mol·L-1 NaC1 was significantly decreased, melanin content in samples treated with 0.4 mol·L-1 NaC1 was significantly decreased in 24 h, but the content in the samples after 48 h treatment was almost the same with the control. Marmitol contents in mycelium cell under different hyperosmotic stress treatments were increased with the increase in time, especially the mannitol content signific

关 键 词：玉米大斑病菌 高渗胁迫 生长发育 渗透调节物质 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治]