微柱凝胶卡式法检测孕妇免疫性抗体效价的应用分析  

Application of microcolumn gel cartridge method in the detection of immunological antibody potency in pregnant women

作　　者：吕小英[1] 陈涌泉[1] 王厚照[1] 李凌波 

机构地区：[1]中国人民解放军第一七四医院检验科,厦门361003 [2]长春博迅生物技术有限责任公司,130102

出　　处：《国际医药卫生导报》2017年第12期1832-1834,共3页International Medicine and Health Guidance News

基　　金：吉林省科技发展计划项目（20140306016YY）

摘　　要：目的探讨微柱凝胶卡式法在产前检测孕妇免疫性抗体效价的作用。方法采用卡式法和试管抗人球蛋白方法平行检0n,0180例0型孕妇（丈夫为A型95例，B型85例）IgG抗-A和抗-B效价，比较二者灵敏度的差异。结果卡式法和试管法检测抗-A效价平均值分别为46．21和33．82，前者是后者1．37倍；抗-B平均效价平均值为47．18和35．02，前者是后者1．35倍。以IgG抗-A（抗-B）效价≥64判为阳性；卡式法和试管法检测方法测IgG抗-A阳性例数分别为45例（47．37％）、8例（8．42％），前者高于后者，差异有统计学意义（自由度=1，x^2=33．23，P〈0．01）；卡式法和试管法检测方法测IgG抗-B阳性例数分别为38例（44．71％）、10例（11．76％），前者高于后者，差异有统计学意义（自由度=1，x^2=24．30，P〈0．01）。结论卡式法应用于产前孕妇免疫性抗体效价检测灵敏度高于试管法，且步骤简便，易于标准化，值得推广使用。Objective To explore the effect of microcolumn gel cassette method in prenatal detection of immunological antibody potency in pregnant women. Methods The anti-A and anti-B potency of 180 cases of O-type pregnant women （95 cases of their partners were A type and the other 85 cases were B type） were detected by both the card method and the test tube anti-human globulin method, and the difference in sensitivity between them were compared. Results The mean value of anti-A potency by the card method and the test tube anti-human globulin method were 46.21 and 33.82 respectively, the former was 1.37 times higher than the latter. The mean value of anti-B potency by the card method and the test tube anti-human globulin method were 47.18 and 35.02 respectively, the former was 1.35 times higher than the latter. To make IgG anti-A （anti-B） potency 〉64 to be positive, the positive cases of IgG anti-A by the card method and the test tube anti-human globulin method were 45 （47.37%） and 8 （8.42%） respectively, the former was higher than the latter, with statistically significant difference （degree of freedom = 1, x^2 = 33.23, P〈0.01）. The positive cases oflgG anti-B by the card method and the test tube anti-human globulin method were 38 （44.71%） and 10 （11.76%） respectively, the former was higher than the latter, with statistically significant difference （degrees of freedom = 1, x^2 = 24.30, P〈0.01）. Conclusion The sensitivity of card method in prenatal detection of the antibody potency of pregnant women is higher than that of test tube anti-human globulin method, with the simple method, and easy to be standardized, worthy to be popularized.

关 键 词：卡式法 试管抗人球蛋白法 免疫性抗体 效价 

分 类 号：R446.6[医药卫生—诊断学] R714[医药卫生—临床医学]