连花定喘片治疗支气管哮喘的疗效  

Efficacy of Lianhua Dingchuan Tablets in bronchial asthma

作　　者：包晨[1] 周霞[2] 冯娜娜[1] 李静 宋元林[1] 白春学[1] 杨冬[1] 周建[1] 

机构地区：[1]复旦大学附属中山医院呼吸科,上海200032 [2]复旦大学附属华山医院呼吸科,上海200040

出　　处：《复旦学报（医学版）》2017年第3期333-338,共6页Fudan University Journal of Medical Sciences

基　　金：北京市科委"十病十药专项"(Z121102001112002);国家自然科学基金(81400018;81570028);高等学校博士学科点专项科研基金(20130071120063);科技部国家重点研发计划(2016YFC1304104)~~

摘　　要：目的评价连花定喘片治疗支气管哮喘的疗效。方法将50只SPF级BALB/C小鼠随机分为对照组、模型组、地塞米松组、连花高剂量组、连花低剂量组,每组10只。采用卵清白蛋白+氢氧化铝致敏,并雾化激发复制小鼠支气管哮喘模型,给药组于每次激发前30 min给药,共计7次。采用特殊气道阻力值评估气道高反应性,肺组织HE染色及肺泡灌洗液(bronchoalveolar lavage fluid,BALF)细胞分类计数评价各组小鼠气道炎症性改变,ELISA法和磁性Luminex~分析法检测BALF和血清中IL-4、IL-13、INF-γ的表达。结果地塞米松组和连花高剂量组能显著降低气道阻力(P<0.05)。地塞米松组和连花高、低剂量组BALF中的各类炎性细胞数量和IL-13的表达均明显减少(P<0.05)。血清IL-13的表达在地塞米松组明显降低(P<0.05),但在连花高、低剂量组中没有变化。各组小鼠BALF和血清中IL-4和INF-γ的表达没有统计学差异。结论连花定喘片可缓解哮喘症状,可能与其降低Th2型细胞因子IL-13的含量,从而减轻气道炎症有关。Objective To evaluate the efficacy of Lianhua Dingchuan Tablets in bronchial asthma. Methods Fifty BALB/C mice were randomly and equally divided into control （Con） group,ovalbumin （OVA） group, dexamethasone （DEX） group, high-dose Lianhua group, low-dose Lianhua group. The mice were sensitized and challenged with OVA plus aluminium hydroxide to establish asthmatic model and were pre-treated 30 minutes before challenge. Specific airway resistance （sRaw） was used to evaluate airway hyperresponsiveness, and airway inflammatory changes were measured. ELISA and Magnetic Luminex were used to quantified the levels of IL-4, IL-13 and INF-γ Results Airway resistance significantly decreased in DEX group and High-dose Lianhua group （P〈0.05）. Levels of inflammatory cells and IL-13 in BALF evidently reduced in DEX group,high-dose Lianhua group and low-dose Lianhua group （P 〈0. 05）, while IL-13 level in serum only decreased in DEX group, There was no significant changes in the levels of IL - 4 and INF -γ among those groups. Conclusions Lianhua Dingchuan Tablets might relieve the symptoms of asthma by reducing IL-13 level and inhibiting the airway inflammation.

关 键 词：连花定喘片 支气管哮喘 气道高反应性 细胞因子 地塞米松 

分 类 号：R562.2[医药卫生—呼吸系统]