新型Rho激酶抑制剂FSD-C10对A53T细胞突起的作用  

The effect of novel Rho kinase inhibitor FSD-C10 on neurite of A53T cell

作　　者：赵静[1,2] 张伟[1,2] 马杰[1,2] 张海飞[2] 尉杰忠[2] 柴智[3] 宋丽娟[3] 肖保国[4] 马存根[1,2,3] 

机构地区：[1]山西医科大学第一临床医学院神经内科,太原030001 [2]山西大同大学脑科学研究所,大同037009 [3]山西中医学院神经生物学研究中心,太原030024 [4]复旦大学华山医院神经病学研究所,上海200025

出　　处：《中国实用神经疾病杂志》2017年第11期1-4,共4页Chinese Journal of Practical Nervous Diseases

基　　金：国家自然科学基金2012年面上项目(81272163);山西省回国留学人员重点科研资助项目(2014-重点7);山西中医学院“2011”培育计划项目(2011PY-1)

摘　　要：目的探讨新型Rho激酶抑制剂FSD-C10对A53T细胞突起的影响及其促进突起生长的机制。方法 A53T细胞进行常规培养和传代,细胞生长到90%左右后,根据不同实验的需要,分为PBS阴性对照组、Fasudil阳性对照组及FSD-C10干预组。应用Image-Pro Plus v6.0软件测量不同时间各组细胞突起的长度,Western blot检测突起相关蛋白synapsinⅠ、synaptophysin、PSD-95的表达情况。结果 FSD-C10促进A53T细胞突起的生长,synapsinⅠ蛋白表达升高。结论 FSD-C10可能通过诱导synapsinⅠ的表达实现对突起生长的促进作用。Objective To investigate the effect of novel Rho kinase inhibitor FSD-C10 on neurite of A53T cell and its possible mechanism of the neurite outgrowth.Methods A53T cells were routinely cultured and divided into three groups：PBS group,Fasudil group and FSD-C10 group.The neurite length of A53T cells was measured by Image-Pro Plus v6.0 at different time points.The expressions of synapsinⅠ,synaptophysin and PSD-95 protein were examined by Western blot.Results Fasudil and PSD-C10 promoted neurite outgrowth of A53T cells.The expression of synapsinⅠ protein in A53T cells was increased,especially in FSD-C10-treated A53T cells.Both Fasudil and FSD-C10 slightly stimulated the PSD-95 at early state,but inhibited it at late state.Conclusion FSD-C10 can promote neurite outgrowth of A53Tcells possibly by its effect on the expression of synapsinⅠ protein.

关 键 词：FSD-C10 A53T细胞 突起 synapsinⅠ 

分 类 号：R33[医药卫生—人体生理学]