基于线粒体CO Ⅰ基因的竹笋夜蛾亲缘关系  

Genetic Relationship of Shoot-Boring Noctuids Based on Mitochondrial Cytochrome Oxidase Ⅰ Gene

作　　者：张琴[1,2] 莫有迪 张亚波[1] 舒金平[1] 王浩杰[1] 吴鸿[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]杭州市林业科学研究院,杭州311300 [3]广东省肇庆市怀集县林业局,肇庆526400

出　　处：《林业科学》2017年第4期96-104,共9页Scientia Silvae Sinicae

基　　金：林业公益性行业科研专项(201304403)

摘　　要：【目的】基于mtDNA CO Ⅰ基因探究竹笋夜蛾的亲缘关系,以确定其分类地位,为系统进化轨迹提供分子理论基础。【方法】以分布于浙江杭州周边竹子产区的4种竹笋夜蛾为研究对象,使用通用引物分别克隆4种竹笋夜蛾线粒体细胞色素C氧化酶亚基Ⅰ(mtDNA CO Ⅰ)基因5'端709 bp片段序列,通过构建系统进化树,分析4种竹笋夜蛾亲缘关系。【结果】1)4种竹笋夜蛾遗传距离较小;2)与比对昆虫相比,4种竹笋夜蛾同属一个进化群,分支支持率达到100%。【结论】4种竹笋夜蛾亲缘关系接近,除萨夜蛾外,建议将其他3种竹笋夜蛾归为一个属。4种竹笋夜蛾形态差异较小,生态习性相似,分布区域基本一致,可作为理想的同域物种进化研究素材。本研究可为4种竹笋夜蛾分类地位的确定提供初步分子基础,同时可为竹笋夜蛾DNA条形码鉴定提供理论基础。[ Objective] The bamboo shoot-boring noctuid is a joint name for several species of moths which are hamful to bamboo shoot stage in southern China. There are four dominant species that have sympatric distribution and the same host. Their biological and ecological characteristics are similar. However, the classification status and evolutionary approach are still controversial. To determine their classification status and provide a molecular theory foundation for the system evolution, their genetic relationship was studied in the paper. [ Method] The bamboo shoot borers, distributing in the bamboo production areas surrounding in Hangzhou, Zhejiang Province, were used as the research object, and four 709 bp segments of cytochrome C oxidase subunit I （CO I ） gene were cloned, using universal primers, from mitochondrial DNA of different bamboo shoot borers＇ individuals and compared with homologous sequences from other lepidopterous insects to analyze their base composition characteristics and the genetic relationship for accurate identification of bamboo shoot borers at molecular level by building system evolutionary trees. [ Result ] 1 ） The results indicated that the genetic distance between the four kinds of bamboo shoot borers was small. 2）Three phylogenetic trees showed that they got 100% support on the branch. [ Conclusion ] The four bamboo shoot borers have closed genetic relationship,among which three species can be classified into one genus except Sapporia repetita. Furthermore, the four kinds of bamboo shoot borers could be used as ideal samples for sympatric speciation research for its similarity in body and habits and distribution. This study provides preliminary molecular basis for the genetic relationship and DNA barcoding technology of the 4 kinds of bamboo shoot moths at the same time.

关 键 词：竹笋夜蛾 CO Ⅰ基因 序列比较 亲缘关系 

分 类 号：S718.7[农业科学—林学]