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机构地区:[1]河北省微生物研究所,保定071051 [2]江西省科学院生物资源研究所,南昌330096
出 处:《植物保护》2017年第3期65-69,96,共6页Plant Protection
基 金:国家自然科学基金(41001150)
摘 要:通过盆栽试验研究了生物有机肥与氯化钾和硫酸钾配施防治香蕉枯萎病的效果。试验结果表明,两种肥料配施促进了香蕉苗生长,降低了香蕉枯萎病病情指数,提高了防病效果,生物有机肥与KCl和K2SO4配施防病效果比单施生物有机肥分别高60%和90%。利用T-RFLP分析土壤细菌DNA多样性,生物有机肥与钾肥配施提高了细菌三个遗传多样性指数,增加了土壤中芽胞杆菌种类。FAME分析也发现生物有机肥以及与钾肥配施促进革兰氏阳性细菌和放线菌繁殖,抑制革兰氏阴性细菌和真菌生长。生物有机肥与钾肥配施,优势互补,改善了土壤微生物群落结构,有利于提高防病效果。The control effects of banana Fusarium wilt(Fusarium oxysporum f.sp.cubense,Foc)by bio-fertilizer(F),and its combination with potassium fertilizers of KCl and K2SO4 were studied.The soil microbial community diversity was investigated by FAME and T-RFLP methods under greenhouse conditions.The results showed that bio-fertilizer and the combinations both promoted plant growth,reduced Fusarium wilt compared to the sterilized bio-fertilizer(control).The combinations of bio-fertilizer with KCl and K2SO4 increased control effect against wilt by 60% and 90% compared to bio-fertilizer treatment alone.The combinations improved soil Bacillus species and bacterial diversity indexes including richness,Shannon diversity and Shannon evenness.FAME results demonstrated that the combinations promoted Gram-positive bacteria and actinomycetes,inhibited Gram-negative bacteria and fungi compared to control and bio-fertilizer treatment alone.The results suggested that the combinations of bio-fertilizer with potassium fertilizer could effectively control banana Fusarium wilt by enhancing the soil bacterial diversity and improving microbial community structure.
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