大麦不同组织成熟过程中DNA甲基化的MSAP分析  

Analysis of Methylation Sensitive Amplification Polymorphism Genome DNA Methylation in Different Barley Tissues during Development

作　　者：熊肖[1,2,3] 李博[1,2,3] 龚强[1,2,3] 徐延浩[1,2,3] 

机构地区：[1]长江大学农学院,湖北荆州434025 [2]湖北省涝渍灾害与湿地农业重点实验室(长江大学),湖北荆州434025 [3]主要粮食作物产业化湖北省协同创新中心(长江大学),湖北荆州434025

出　　处：《长江大学学报（自然科学版）》2017年第10期29-33,42,共6页Journal of Yangtze University(Natural Science Edition)

基　　金：长江大学青年基金资助项目(2015cqr16);湿地生态与农业利用教育部工程中心开放课题(KF201601)

摘　　要：DNA甲基化参与调控生长植物发育。使用甲基化敏感扩增多态性(methylation sensitive amplification polymorphism,MSAP)方法并结合毛细管自动电泳分析仪,对大麦(Hordeum vulgare L)的种子、根、茎、叶等4种组织在成熟过程中的DNA甲基化修饰进行了分析。结果表明,大麦不同组织在成熟过程中基因组总甲基化水平改变较小,但44.90%~65.17%的CCGG位点甲基化模式在不同组织在成熟过程中发生了改变。在大麦种子成熟过程中,CCGG位点半甲基化水平急剧升高,达到65.40%,全甲基化位点显著下降仅为13.39%,与其他组织的甲基化特征差异显著。结果显示,在大麦组织成熟的过程中,DNA甲基化修饰发生了巨大的变化。DNA methylation is one of the mechanism in controlling plant development.Methylation sensitive amplification polymorphism(MSAP)approach was used to investigate the DNA methylation changes in barley seed,root,stem and leaf from juvenile to mature combined with automatical capillary electrophoresis analyzer.The results showed that the genome methylation level during development in different tissues maintained stability.However,about 44.90% to 65.17% of the detected CCGG sites showed methylation changes during development from juvenile to mature.The hemi methylation level in the matured barley seed reached to 65.40% and the full methylation level was only 13.39%,which displayed a specific DNA methylation pattern compared with the other tissues.It revealed that the DNA methylation changes during barley tissues from juvenile to mature.

关 键 词：大麦(Hordeum vulgare L) DNA甲基化 甲基化敏感扩增多态性(MSAP) 组织类型 发育 

分 类 号：Q78[生物学—分子生物学] S512.3[农业科学—作物学]