微囊藻毒素3种异构体的提取净化研究  

Study on the Extraction and Purification of 3 Isomers of Microcystins

作　　者：李莉 蔡金傍[2] 赵志强[2] 孙旭[2] LI Li CAI Jin-bang ZHAO Zhi-qiang et al(Putian Environmental Monitoring Station, Putian, Fujian 351100 Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing, Jiangsu 210042)

机构地区：[1]福建省莆田市环境监测中心站,福建莆田351100 [2]环境保护部南京环境科学研究所,江苏南京210042

出　　处：《安徽农业科学》2017年第15期51-53,共3页Journal of Anhui Agricultural Sciences

基　　金："十二五"村镇建设领域国家科技计划课题(2014BAL02B04)

摘　　要：[目的]建立微囊藻毒素提取净化的方法。[方法]对比分析固相萃取柱、及洗脱液等对微囊藻毒素3种异构体(MC-LR、MCYR、MC-RR)提取净化的影响,并建立固相萃取提取净化微囊藻毒素的方法。[结果]Accu BOND II ODS C18固相萃取柱对微囊藻毒素中3种异构体具有较高的回收率,其回收率均达80%以上;甲醇浓度越低的淋洗液淋洗能力越差,10%甲醇能去除大部分色素杂质,且3种异构体的流失率较小;甲醇浓度越高的洗脱液洗脱能力越强,70%和80%较低浓度甲醇洗脱液,其洗脱收集液中杂质较少,其中80%甲醇是较理想的洗脱液。[结论]该研究建立的微囊藻毒素提取净化方法简单可行,可为今后的工业化应用提供科学依据。[ Objective] To establish method for extracting and purifying microcystins. [ Method] The effects of solid phase extraction column, eluent on Extraction and purification of 3 isomers of microcystins （ MCs, LR, YR and RR） were analyzed and compared. A method for extraction and purification of MCs by solid phase extraction was established. [ Result] The AccuBOND II ODS C18 solid phase extraction column has a high recovery rate for 3 isomers in MCs, and the recovery rate was above 80%. The lower the methanol concentration was, the worse the leaching capacity of the eluent, and 10% methanol leaching can remove most of the pigment impurities, and the loss rate of the 3 isomers was smaller. The higher the methanol concentration was, the stronger the elution capacity was, and the lower concentration of methanol eluent was 70% and 80%. There was less impurity in the eluent collection liquid, and 80% of the methanol was an ideal eluent. [ Conclusion]The method of extraction and purification of Microcystis was simple and feasible, which can provide scientific basis for the industrialization application in the future.

关 键 词：微囊藻毒素 固相萃取 提取 净化 回收率 

分 类 号：X52[环境科学与工程—环境工程]