两种方法检测鼠疫耶尔森菌Pla片段的研究  

Detection of Pla Fragment in Yersinia pestis by two methods

作　　者：靳木子 云华 邰大鹏[2] 陈宇飞 白潇 JIN Mu-zi YUN Hua TAI Da-peng CHEN Yu-fei BAI Xiao(Inner Mongolia International Health Care Center, Hohhot 010020, Inner Mongolia Autonomous Region, China Baotou Medical College Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People＇s Republic of China Scientific Research Subject （No. 2015IK176） and the Natural Science Foundation of Inner Mongolia Autonomous Region of China （No. 2015BS0805)

机构地区：[1]内蒙古国际旅行卫生保健中心,呼和浩特010020 [2]包头医学院,内蒙古包头014040

出　　处：《中国媒介生物学及控制杂志》2017年第3期238-240,共3页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局科技计划项目(2015IK176);内蒙古自然科学基金(2015BS0805)~~

摘　　要：目的研究Southern印记杂交方法检测鼠疫耶尔森菌(鼠疫菌)Pla片段的有效性。方法采用Southern印记杂交技术与鼠疫菌核酸测定试剂盒两种方法对鼠疫菌Pla片段进行检测。结果两种方法检测结果相同,Southern印记杂交技术可直观地观察pla基因拷贝数,杂交效率较高;实时荧光定量PCR检测结果为阳性。结论 Southern印记杂交技术适用于鼠疫菌Pla片段的检测。Objective To determine the effectiveness of Southern blot method for detection of Pla fragment in Yersinia pestis. Methods Using Southern blot and Y. pestis quantitative real-time PCR kit to detect the Pla fragment. Results The results of Southern blot were same as real-time PCR kit. Southern blot allowed to see the number of pla gene copies directly, the efficiency of hybridization was high; PCR detection was also positive. Conclusion Southern blot is suitable for detection of Pla fragment of Y. pestis.

关 键 词：Southern印记杂交 实时荧光定量聚合酶链式反应 鼠疫耶尔森菌 

分 类 号：R254.8[医药卫生—中医内科学]