嗜吞噬细胞无形体在不同组织细胞间抗原差异研究  

Analysis of antigenic diversity of Anaplasma phagocytophilum grown in different cells

作　　者：高娃[1] 殷旭红[1] 郭生春 乌兰图雅[1] 曹民治 Gaowa YIN Xu-hong GUO Sheng-chun Wulantuya CAO Min-zhi(Department of Medicine, Hetao College, Bayannur 015000, Inner Mongolia Autonomous Region, China Byannur Center for Disease Control and Prevention Supported by the Science and Technology Project of Bayannur, Inner Mongolia Autonomous Region and Scientific Research Foundation for Introduced Talents, Hetao College, Inner Mongolia Autonomous Region)

机构地区：[1]河套学院医学系,内蒙古巴彦淖尔015000 [2]巴彦淖尔市疾病预防控制中心,内蒙古巴彦淖尔015000

出　　处：《中国媒介生物学及控制杂志》2017年第3期255-257,共3页Chinese Journal of Vector Biology and Control

基　　金：巴彦淖尔市科技计划;河套学院引进人才科研启动基金~~

摘　　要：目的调查嗜吞噬细胞无形体在不同组织细胞间产生抗原差异的原因。方法采用RT-PCR法对嗜吞噬细胞无形体在不同组织细胞中的抗原蛋白表达差异进行分析,并利用真核无细胞蛋白表达系统合成重组蛋白,利用蛋白质印迹法进行测试。结果组织培养细胞不同,嗜吞噬细胞无形体菌体表面可产生不同的P44外膜蛋白。通过检测发现,3种r P44蛋白易与患者血清中抗体结合,可作为人粒细胞无形体病的诊断用抗原。结论不同组织细胞间存在抗原性差异的不同嗜吞噬细胞无形体。Objective To analyze the reasons for antigen diversity ofAnaplasma phagocytophilum grown in different cells. Methods The P44 transcripts were analyzed using nested RT-PCR, and the P44 proteins were produced as recombinant protein in vitro by insect cell-free protein synthesis system. Results We detected and characterized P44 transcript variants of A. phagocytophilum from THP-1 and HL60 ceils. And our study demonstrated the presence of specific antibodies against the 3 type of rP44 proteins that prevailed in infected THP-1 and HL60 ceils, probably being suitable as protein antigens for sero-diagnosis of human granulocytic anaplasmosis. Conclusion We demonstrated antigenic diversity of a variety of A. phagocytophilum grown in different ceils.

关 键 词：嗜吞噬细胞无形体 P44外膜蛋白 无形体病 

分 类 号：R376[医药卫生—病原生物学]