Th17、Th22/Treg细胞及相关细胞因子在儿童过敏性紫癜患者外周血中变化及意义  

Significance and changes of Th17,Th22 and Treg cells in peripheral blood of children patients with Henoch-Schonlein purpura

作　　者：邢超[1] 邵美娟[1] 陈慧[1] 宫剑[1] 陈锴新 杨军军[1] XING Chao SHAO Mei-juan CHEN Hui GONG Jian CHEN Kai- xin YANG Jun-jun(Clinical Laboratory, the Second Affiliated Hospital ＆ Yuying Children＇s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, Chin)

机构地区：[1]温州医科大学附属第二医院育英儿童医院检验科,浙江温州325000 [2]温州医科大学检验与生命科学院,浙江温州325000

出　　处：《中国卫生检验杂志》2017年第10期1432-1434,共3页Chinese Journal of Health Laboratory Technology

基　　金：温州市科技局项目(Y20140280)

摘　　要：目的观察儿童过敏性紫癜(HSP)患儿初发及缓解期Th17、Th22及Treg细胞频数及相关细胞因子水平变化,以探讨HSP发病的免疫学机制。方法收集本院2014年-2016年收治的HSP初发患儿68例HSP初诊组,缓解期患儿68例为HSP缓解组,另选择同期健康儿童35例为健康对照(HC组)组,流式细胞仪检测3组外周血Th17、Th22细胞及Treg细胞频数;ELISA法检测患儿血浆中细胞因子IL-17、IL-35、IL-22及IL-6。结果 HSP初诊组外周血Th17、Th22细胞比例较HC组、HSP缓解组均明显升高,差异均有统计学意义(P<0.05);Treg细胞比例较HC组、HSP缓解组均明显下降;HSP初诊组细胞因子IL-17、IL-22、IL-35、IL-6较HSP缓解组及HC组均明显升高,上述差异均有统计学意义(P<0.05)。结论 Treg细胞频数下降、Th22、Th17等促炎细胞及相关细胞因子高表达可能是HSP发生的免疫学机制。Objective To observe the frequencies of Th17, Th22 and Treg cells and the level changes of related cytokines in primary and remission stages of pediatric Henoch -Schonlein purpura（HSP） , so as to investigate the role of immune pathogene- sis. Methods 68 HSP incipient patients, 68 remission patients aclmitted in our hospital during 2014 -2016 were selected as the primary group and the remission group, respectively; meanwhile, 35 eases for healthy physical examination were selected as the healthy control group ; flow cytometer was used to determine the frequencies of Th17, Th22 cells and regulatory T cells in the peripheral blood of the 3 groups, and ELISA was used to determine the related cytokines including IL - 17, IL- 35, IL -22 and IL -6 in plasma of patients in 3 groups. Results The frequencies of Th17 cells and Th22 cells in HSP incipient group were higher than those in HSP ease group and HC group, and the differences were of statistical significance（ P 〈 0.05 ） ; the fre- quencies of Treg cells in HSP declined obviously than those in HSP ease group and HC group; IL - 17, IL -22, IL -35, IL -6 in HSP group were higher than those in HC and HSP ease groups, and the differences were statistically significant（ P 〈 0.05 ）. Conclusion Decreasing of Treg cells, over - expression of Th22 cells and Th17 cells and related cytokines may cause the im- mune pathogenesis of HSP

关 键 词：过敏性紫癜 Th17细胞 调节T细胞 TH22细胞 细胞因子 

分 类 号：R725.5[医药卫生—儿科]