集合丙型肝炎病毒-RNA RT-PCR方法对宁夏美沙酮门诊人群丙型肝炎年发病率的预估研究  

Estimation for annual incidence rate of HCV by pooled HCV-RNA RT-PCR in Ningxia

作　　者：鱼小红[1] 吴忠兰[1] 关光玉[1] 赵伟明[2] 曹敏[1] 尹婷[2] YU Xiao -hong WU Zhong- lan GUAN Guang- yu ZHAO Wei -ming CAO Min YIN Ting(Ningxia Center for Disease Control and Prevention, Yinchuan, Ningxia 750004, Chin)

机构地区：[1]宁夏疾病预防控制中心,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《中国卫生检验杂志》2017年第10期1440-1441,1444,共3页Chinese Journal of Health Laboratory Technology

基　　金：宁夏科技攻关计划项目国际合作专项(2013ZYH193);宁夏自然科学基金(NZ13217)

摘　　要：目的通过集合HCV-RNA RT-PCR的方法,对宁夏丙型肝炎年发病率估算。方法采用50∶1、10∶1、1∶13级集合HCV-RNA RT-PCR方法及用于Pooling估计丙型肝炎年发病率的公式进行发病率估计。结果收集血浆共1 210份,2次采用酶联免疫吸附法(ELISA)检测丙型肝炎病毒(HCV)抗体阳性170例,抗体阳性率为14.1%;集合检测HCV抗体阴性样本共计1 040例,最后检测到HCV-RNA阳性样本36例,丙型肝炎病毒(HCV)年发病率为5.1%~8.7%。结论采用Pooilng方法对高危人群发病率预估是行之有效的。Objective To estimate annual incidence rate of HCV in Ningxia by pooled HCV RNA RT - PCR. Methods Pooled HCV RNA RT - PCR of 3 - staged pooling was taken by 50 ： 1. 10 ： 1 and 1 ： 1 based on the formula to estimate incidence of hepatitis C virus（HCV）. Results 1 210 cases of plasma were collected, 170 cases were the antibody positive in two detections by enzyme - linked immunosorbent assay（ ELISA）, and the positive rate of antibody was 14.1% ; 1 040 cases of negative HCV antibody were detected, and 36 cases were HCV - RNA positive, the incidence rate of annual HCV was within 5. 1% - 8.7%. Conclusion It confirms that estimation for the incidence of high - risk population by pooed HCV RNA RT - PCR is effective.

关 键 词：集合HCV-RNA RT-PCR 年发病率 丙型肝炎 估算 

分 类 号：R512.63[医药卫生—内科学]