泰国输华真柏中一种隐皮孢囊线虫的鉴定  

Diagnosis of Cryphodera sp. intercepted on Juniperus chinensis from Thailand

作　　者：龙海[1,2] 齐小峰[1] 谢泳桂 李一农[1] 李芳荣[1] 

机构地区：[1]深圳出入境检验检疫局,广东深圳518001 [2]深圳市检验检疫科学研究院深圳市外来有害生物检测技术研发重点实验室

出　　处：《植物检疫》2017年第3期29-32,共4页Plant Quarantine

摘　　要：深圳口岸从来自泰国真柏苗木中截获一种隐皮孢囊线虫。序列分析显示截获的孢囊线虫28S r DNA-D2/D3区基因序列与Gen Bank中布林克曼隐皮孢囊线虫(Cryphodera brinkmani)(登录号为JQ965677和DQ328705)的序列相似性为99%。系统进化树中截获的孢囊线虫和布林克曼隐皮孢囊线虫聚为一个分支。结合形态学特征比较和分子特征分析,将截获的孢囊线虫鉴定为布林克曼隐皮孢囊线虫。这是我国口岸首次报道截获该线虫。The Cryphodera sp. was detected in roots of Juniperus chinensis from Thailand at Shenzhen port. 28S rDNA-D2/D3 region sequence analysis showed 99% identity with Cryphodera brinkmani in Gen- Bank （accession No. JQ965677 and DQ328705）. The phylogenetic tree based on 28S rDNA-D2/D3 region revealed that the intercepted nematode clustered with C.brinkmani. According to the results of morpholog- ical and molecular characteristics,the intercepted cyst nematode was identified as C.brinkmani. This is the first report of its interception in China.

关 键 词：布林克曼隐皮孢囊线虫 真柏 泰国 形态学 分子鉴定 

分 类 号：S763[农业科学—森林保护学]