牛大力多糖对小鼠常压耐缺氧的研究  

Study on mice's normobaric hypoxia tolerance of polysaccharides from Millettia speciosa Champ.

作　　者：唐专辉[1] 罗秋莲[1] 莫静[1] 张雪凤[1] 朱艺萍[1] 李燕婧[2] 李典鹏 黄江燕[4] 罗轩[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]广西中医药研究院,广西南宁530022 [3]广西植物研究所广西植物功能物质研究与利用重点实验室,广西桂林541006 [4]中国人民解放军第181医院广西代谢性疾病研究重点实验室,广西桂林541002

出　　处：《广西大学学报（自然科学版）》2017年第3期1203-1208,共6页Journal of Guangxi University（Natural Science Edition）

基　　金：广西自然科学基金青年基金资助项目(2013GXNSFBA019024);广西植物功能物质研究与利用重点实验室开放基金资助项目(FPRU2011-4);广西代谢性疾病研究重点实验室开放基金资助项目(181H2011-02);广西大学2011级大学生实验技能和科技创新能力训练基金资助项目(SYJN20130303)

摘　　要：以牛大力(Millettia speciosa Champ.)为对象,利用小鼠常压耐缺氧为实验模型,研究牛大力多糖的抗疲劳作用。实验将小鼠随机分为10组,分别为空白对照组、阳性药对照组(人参蜂王浆,7 m L/kg)和8个给药组,每组10只,雌雄各5只。对应的给药组分别为牛大力多糖低分子量段1低、高浓度组[100 mg/(kg·d)、200 mg/(kg·d)];牛大力多糖低分子量段2低、中、高浓度组[50 mg/(kg·d)、100 mg/(kg·d)、200 mg/(kg·d)];牛大力多糖高分子量段1&2低、中、高浓度组[50 mg/(kg·d)、100 mg/(kg·d)、200 mg/(kg·d)]。连续灌胃给药14 d后,考察其对小鼠常压耐缺氧时间的影响。结果表明:与空白对照组比较,各个给药组都能一定程度的延长小鼠常压耐缺氧时间,并且呈现出明显的剂量效应。其中牛大力多糖高分子量段1&2低浓度组的效果最好,高于阳性药对照组的药效。In this paper,the anti-fatigue effect of polysaccharides from Millettia speciosa Champ.( NDLs) was studied using models of mice normobaric hypoxia tolerance. Mice were dividedrandomly into ten groups,a blank control group,a positive control group( ginseng royal jelly,7 m L/kg) and eight treatment groups. Each group had ten mice,five females and five males. The treatment groups included low-molecular weight polysaccharides part 1 at low and high doses[100,200 mg/( kg·d) ],low-molecular weight polysaccharides part 2 at low,medium and high doses[50,100,200 mg/( kg·d) ],and high-molecular weight polysaccharides part 1&2 at low,medium and high doses[50,100,200 mg/( kg·d) ],respectively. All groups were treated by gavage for 14 consecutive days. The normobaric hypoxia tolerance time was determined to investigate the influence of polysaccharides from NDLs on mice. The results indicated that all treatment groups can somewhat prolong the normobaric hypoxia tolerance time and exhibited an obvious dose-dependent manner compared with the blank control group. Among which,the effect of high-molecular weight polysaccharides part 1&2 at low dose group was the best and superior to that of positive control group.

关 键 词：牛大力 多糖 常压耐缺氧 抗疲劳 

分 类 号：S858.23[农业科学—临床兽医学]