有氧运动联合褪黑素对Ⅱ型糖尿病大鼠骨质疏松的影响  

Effects of aerobic exercise combined with melatonin on osteoporosis of type Ⅱ diabetic rats

作　　者：景会锋[1] 王小梅[1] JING Hui-feng WANG Xiao-mei(Department of Physical Education, Yanan University, Yanan 716000, China)

机构地区：[1]延安大学体育学院,陕西延安716000

出　　处：《中国应用生理学杂志》2017年第3期252-256,共5页Chinese Journal of Applied Physiology

基　　金：2015年陕西省体育局常规课题项目(15035)

摘　　要：目的:观察有氧运动和褪黑素对Ⅱ型糖尿病大鼠骨质疏松的影响。方法:6周龄的成年雌性SD大鼠60只,随机分为安静对照组(N组)10只和Ⅱ型糖尿病模型组50只,N组大鼠不加任何干预,Ⅱ型糖尿病模型组大鼠一次性腹腔注射35 mg/kg链脲佐菌素(STZ),1周后检测大鼠血糖大于16.7 mmol/L为Ⅱ型糖尿病造模成功,将40只成模大鼠随机分为糖尿病对照组(D)、糖尿病+有氧运动组(DE)、糖尿病+褪黑素组(DM)、糖尿病+有氧运动+褪黑素组(DEM),每组10只;DE组和DEM组大鼠采用20 min的递增负荷的方式进行跑台有氧运动,训练持续6周,DM组和DEM组大鼠每天灌胃40 mg/kg褪黑素,观察各组大鼠体重、脊椎骨以及左右股骨骨密度(BMD)、观察大鼠血糖、血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、血清总钙(Ca)、无机磷(P)和甲状旁腺素(PTH)的变化。结果:与N组相比,D组大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著降低(P<0.05,P<0.01),血糖、血清MDA和血PTH水平显著升高(P<0.01),血P无明显变化(P>0.05);与D组比较,DE组、DM组大鼠大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著升高(P<0.05,P<0.01),血糖、血清MDA和血PTH水平显著降低(P<0.05,P<0.01),血P无明显变化(P>0.05),有氧运动和褪黑素同时干预效果更好。结论:有氧运动和褪黑素均能改善糖尿病骨质疏松,且两者联合干预的效果更加显著,其可能与通过提高糖尿病大鼠的抗氧化应激能力,调节糖的代谢从而有效地降低血钙和PTH,改善BMD来缓解骨质疏松有关。Objective： To investigate the effects of aerobic exercise and melatonin on osteoporosis in type 2 diabetic rats. Methods： Sixty female SD rats were randomly divided into the control group （ n = 10） and the diabetic model group （ n = 50）. The rats in the control group（ N group） did not receive any intervention, and the rats in the type [][ diabetic group （D） was injected with streptozocin（STZ） at the dose of 35 mg/kg by intraperitoneally. One week later, rats with blood glucose greater than 16.7 mmol/L were successfully modeled for type [1 diabetes. Forty diabetic rats were randomly divided into diabetic control group （D）, diabetes ＋ aerobic exercise group（CDE）, diabetes ＋ melatonin group （DM）, diabetes mellitus ＋ aerobic exercise ＋ melatonin group （DEM）, 10 rats in each group. The rats in DE and DEM groups were treated with 20 min aerohic exercise for 6 weeks. The rats in DM group and DEM group were given 40 mg/kg of melatonin per day. The body weight, vertebrae and left and right femur bone mineral density （BMD） of the rats in each group were observed. Malondialdehyde（MDA）, supemxide dismutase （SOD）, glutathione peroxidase （ GSH-Px）, serum total calcium （Ca）, inorganic phosphate （P） and parathyroid hormone （PTH） were detected. Results： Compared with N group, body weight, serum SOD, GSH-Px levels, blood Ca, lumbar and femoral BMD of rats in group D were decreased significantly （ P 〈 0.05 or P 〈 0.01 ）. The levels of blood glucose, serum MDA and serum PTH were increased signifificantly （ P 〈 0.01 ）, the level of blood P had no changes （ P 〉 0.05） ; compared with group D, DE group, DM group rats hody weigbt, sermn SOD, GSH-Px levels, blood Ca, lumbar spine and femur about BMD were increased significantly （ P 〈 0.05 or P 〈 0.01 ）. The levels of blood glucose, serum MDA and serum PTH were decreased significantly （P 〈 0.05 or P 〈 0.01 ）, the level of blood P had no changes （P 〉 0.05）. The effects in

关 键 词：有氧运动 褪黑素 二型糖尿病 骨质疏松 

分 类 号：G804.7[文化科学—运动人体科学]