尿刊酸修饰纳米壳聚糖载体负载NGAL siRNA干扰结肠癌细胞NGAL基因的实验研究  

The experimental research of Neutrophil Gelatinase-associated Lipocalin（NGAL） siRNA encapsulated by UAC on NGAL gene silence in colon cancer cells

作　　者：张秀峰[1] 王厚东 沈忠[1] 杨关根[1] 潘洁莉[2] 李美芽 ZHANG Xiu-feng WANG Hou-dong SHEN Zhong YANG Guan-gen PAN Jie-li LI Mei-ya(Department of Coloproctology, The Third Peoples＇ Hospital of Hangzhou, Hangzhou 310009, China Center of forecasting and analysis, Zhejiang Chinese Medical University, Hangzhou 310009, China.)

机构地区：[1]杭州市第三人民医院(安徽医科大学杭州临床学院)肛肠外科,浙江杭州310009 [2]浙江中医药大学分析测试中心,浙江杭州310009

出　　处：《中国生化药物杂志》2017年第5期14-17,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：浙江省科技厅公益项目(2013C33210);浙江省卫生厅骨干人才项目(2013RCB013);杭州市科技局重点专科专病项目(20140733Q20)

摘　　要：目的探讨尿刊酸修饰壳聚糖负载siRNA对结肠癌细胞NGAL基因的沉默效应。方法采用对照组、尿刊酸修饰壳聚糖（UAC）组及壳聚糖（CTS）组分别包裹NGAL siRNA转染结肠癌细胞株HT-29,采用ELISA法检测NGAL蛋白水平。结果经转染siRNA后,ELISA法测得NGAL分泌蛋白量,UAC组为0.583μg/L,显著低于CTS组0.772μg/L和对照组1.071μg/L（P〈0.05）。结论尿刊酸修饰壳聚糖携载小干扰RNA可以明显下调HT29结肠癌细胞株NGAL基因和蛋白的表达。Objective To explore the effect of NGAL knockdown by NGAL siRNA encapsulated with urocanic acid-modified chitosan nanoparticles （UAC）.MethodsNGAL siRNA encapsulated by UAC and chitosan （CTS） respectively, which were then used to transfect human colon cancer cell lines HT29.The expression level of NGAL protein were detected by Enzyme Linked Immunosorbent Assay（ELISA）.ResultsThe ELISA study revealed that the expression level of NGAL protein in UAC group（average 0.583μg/L） was significantly lower than in CTS group （average 0.772μg/L） and control group（average 1.071μg/L） （P〈0.05）.ConclusionThe NGAL expression of mRNA and protein in HT29 cells could be down-regulated by siRNA encapsulated by UAC.

关 键 词：结肠癌 NGAL 壳聚糖 RNA干扰 

分 类 号：R3[医药卫生—基础医学]