黄芩苷对黏菌素致小鼠周围神经毒性的保护作用研究  

Baicalin Protects Against Colistin-induced Peripheral Neuro Toxicity in Mice

作　　者：陆子音[1] 连凯霞[1] 李继昌[1] LU Zi-yin LIAN Kai-xia LI Ji-chang(Northeast Agricultural University College of Animal Medicine, Harbin 150030, China)

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2017年第4期13-16,19,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31472240)

摘　　要：黏菌素的神经毒性是众所周知的。中药黄芩苷具有抗氧化、保护神经等作用。本试验通过设定正常组(生理盐水)、模型组(静脉注射15 mg/kg体重黏菌素)、黄芩苷高剂量组(200 mg/kg体重黄芩苷+15 mg/kg黏菌素)、黄芩苷中剂量组(100 mg/kg体重黄芩苷+15 mg/kg体重黏菌素)和黄芩苷低剂量组(50 mg/kg体重黄芩苷+15 mg/kg体重黏菌素)共5个剂量组,连续给药7 d后,进行氧化-抗氧化相关生化指标测定(MDA、SOD、CAT和GSH)、坐骨神经组织中凋亡相关因子表达(Caspase3和Bax)及碱性髓鞘蛋白(myelin basic protein,Mbp)表达的测定。结果表明,黄芩苷干预组的小鼠氧化指标明显降低,提高小鼠的抗氧化能力;Caspase3和Bax以及Mbp蛋白表达量均有降低。Colistin-induced neurotoxicity is well - known. Baicalin has a lot of pharmacological effects, such as antioxidation, neuro-protection, et al. In this Study, mice were divided into five dose groups： normal group （saline）, model group （intravenous colistin 15 mg/kg）, baiealin high close group （200 mg/kg baicalin ＋ 15 mg/kg colistin）, and baicalin media dose group （ 100 mg/kg baicalin ＋ 15 mg/kg colistin）, baicalin low dose group （50 mg/kg baicalin ＋ 15 mg/kg colistin）. After 7 days of administration, we measured the oxidation-antioxidant, expression of apoptosis-related factors （caspase3 and Bax） and myelin basic protein detection in sciatic nerve. The results showed that： baicalin significantly reduced reactivity of oxygen indicators and improved the anti-oxidative capacity The expression of Caspase3, Bax and Mbp proteins were significantly decreased by baicalin.

关 键 词：黄芩苷 黏菌素 神经毒性 

分 类 号：S852[农业科学—基础兽医学]