可筛选抗耳毒性化合物的斑马鱼模型的建立  

Establishment of the Zebrafish Model for Screening Anti-ototoxic Compounds

作　　者：万伟华[1] 黄文俪 温露[1] WAN Weihua HUANG Wenli WEN Lu(School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China)

机构地区：[1]广东药科大学药学院,广东广州510006

出　　处：《今日药学》2017年第5期312-315,共4页Pharmacy Today

摘　　要：目的建立一种快速筛选抗耳毒性化合物的斑马鱼毛细胞损伤模型。方法将受精后5 d(5 dpf)的斑马鱼幼鱼置于庆大霉素(10,25,50,100μmol·L^(-1))和DMSO溶液(0.2%,0.5%)中孵育不同的时间。DASPEI染色后,荧光显微镜下对神经丘(SO1、SO2、O1、OC1)中毛细胞进行观察和计数。结果 10μmol·L^(-1)庆大霉素溶液中孵育6 h达到近IC50效果,且0.2%DMSO对斑马鱼的毒性低。结论斑马鱼毛细胞损伤模型建立成功,可用于快速大规模筛选抗耳毒性化合物。ABSTRACT：OBJECTIVE To establish a model of hair cell damage for rapid fishing of anti-ototoxic compounds. METHODS Five days post-fertilization （5 dpf） zebrafish larvae were exposed respectively to gradient concentrations of gentamicin （ 10,25,50,100 μmol. L-1） and DMSO （0.2%,0.5%） for different time periods.Then hair cells within the four neuromasts （SO 1, SO2, O1, OC1 ） were observed by fluorescence microscopy and the number of the hair cells was counted after stained with DASPEI. RESULTS Incubation in 10 μmol. L-1 gentamicin solution for 6 h could nearly achieve the effect of ICs0 ,and 0.2% DMSO had an effect on zebrafish with low toxicity. CONCLUSION The zebrafish model of hair cell damage is successfully established and can be used for drug screening in a large scale quickly.

关 键 词：听力损伤 斑马鱼 庆大霉素 抗耳毒性化合物 

分 类 号：R965.1[医药卫生—药理学]