不同保存处理对尿标本AD7C-NTP检测结果的影响研究  

Influence of different storage ways on the testing results of AD7C-NTP in urine specimen

作　　者：许程洁[1] 王智斌[1] 张杰[1] 唐义斌[1] 谭泰昌[1] 

机构地区：[1]四川省医学科学院/四川省人民医院检验科,四川成都610072

出　　处：《国际检验医学杂志》2017年第11期1447-1448,1451,共3页International Journal of Laboratory Medicine

基　　金：四川省科技厅科技计划项目(30504010207)

摘　　要：目的探讨不同保存条件和保存时间对尿液标本阿尔茨海默病(AD)相关神经丝蛋白(AD7C-NTP)检测结果的影响。方法于2015年10月至2016年1月,选取AD确诊患者50例,留取尿液标本,按不同保存条件分为室温保存组、4℃保存组、-20℃保存组,分别于不同时间检测AD7C-NTP水平;同时在4℃保存组和-20℃保存组分别加入防腐剂,在不同时间检测AD7C-NTP水平。结果室温保存组和4℃保存组标本保存3d内,检测结果与初始检测值比较差异无统计学意义(P>0.05);7d后,4℃保存组检测结果与初始检测值比较差异无统计学意义(P>0.05);-20℃保存组保存1d后,检测结果与初始检测值比较差异有统计学意义(P<0.05)。加入防腐剂后,4℃保存组检测结果稳定性不及未加防腐剂组,-20℃保存组检测结果过稳定性增加,但检测偏差仍超过可接受范围。结论 4℃不加防腐剂是用于检测AD7C-NTP水平的尿标本最理想保存条件。Objective To explore the influence of different preservation conditions and time period on the testing results of Alzheimer′s disease(AD)associated neuronal thread protein(AD7C-NTP)in urine specimen.Methods From Oct.2015 to Jan.2016,urine specimen were collected from 50 AD patients,and divided into three groups,according to the different storage temperature,including room temperature group,4℃ group and-20℃ group.Preservatives were added into specimen of 4℃ preservation group and-20 ℃ preservation group.AD7C-NTP level was detected at different preservation time of all specimen.Results The testing results of AD7C-NTP in specimen of room temperature group and 4 ℃ groups,detected within three days,were not significantly different with initial detection value(P>0.05).After seven days,the testing results in specimen of 4 ℃ group were not significantly different with initial detection value(P>0.05).However,after one day,the testing results in specimen of-20 ℃ group were significantly different with initial detection value(P<0.05).Contrast with 4℃ without preservative group,the adding of preservative could not increase the stability of AD7C-NTP.The adding of preservative in specimen of-20℃could obviously increase stability,but the deviation of testing results was beyond acceptable limits.Conclusion 4℃ without preservatives could be the optimal storage condition for detection of AD7C-NTP in the urine.

关 键 词：神经丝蛋白 阿尔茨海默 保存 尿 

分 类 号：R446.12[医药卫生—诊断学] R749.16[医药卫生—临床医学]