乳酸-钴(Ⅱ)-1,10-菲啰啉三元配合物与DNA结合的光谱与电化学研究  

Spectroscopic and Electrochemical Investigation on the DNA-binding Property of a Cobalt(Ⅱ) Complex with Mixed-ligands of Lactic Acid and 1,10-Phenathroline

作　　者：邱玮玮[1] 王庆华[1] 林海彬[1] 汪庆祥[1] 

机构地区：[1]闽南师范大学化学与环境学院,福建漳州363000

出　　处：《化学试剂》2017年第6期573-577,582,共6页Chemical Reagents

基　　金：国家自然科学基金资助项目(21275127);福建省教育厅基金资助项目(JA14195);福建省教育厅中青年教师教育科研项目(JA15305)

摘　　要：以1,10-菲啰啉(phen)和乳酸为配体合成了三元钴配合物[Co(phen)(la)_2]。采用紫外光谱、循环伏安法、计时库仑法和微分脉冲伏安法等考察了其与鲱鱼精天然DNA的相互作用。紫外光谱实验显示,在[Co(phen)(la)_2]中加入DNA后,配合物在270 nm处的紫外特征吸收峰出现明显的减色效应,并伴随红移,表明[Co(phen)(la)_2]可通过其phen配体插入DNA中相邻碱基对。循环伏安法和计时库仑法进一步表明,与DNA结合后,复合物在水溶液中扩散系数降低,从而导致电化学信号减弱。微分脉冲伏安实验显示,[Co(phen)(la)_2]氧化峰电流与DNA浓度在2.66×10^(-5)~1.34×10^(-4)mol/L范围内呈现良好的线性关系,表明[Co(phen)(la)_2]可作为一种潜在的DNA检测探针。A ternary cobalt complex [ Co（phen） （la） 2 ] has been synthesized by the reaction of cobalt salt with 1,10-phenanthro- line （phen） and lactic acid. The interaction of the complex with natural fishsperm DNA was investigated by UV absorbance spec- troscopy,cyclic vohammetry,chronocoulometry and differential pulse vohammetry. The results of UV absorbance spectroscopy at 270 nm revealed that DNA could induce the hypochromic effect and red-shift of characteristic absorbance peak of the complex, suggesting that the cobalt complex can interact with DNA via a typical intercalation binding mode. The cyclic vohammetry and chronocoulometry further showed that the diffusion coefficient of the complex was decreased after the interaction of the complex with DNA. Quantitative analysis by differential pulse voltammetry demonstrated that the cobalt complex can be successfully used for the detection of DNA as an electrochemical probe,with a linear response range of 2.66 × 10 ^-5 - 1.34 × 10 ^-4 mol/L.

关 键 词：钴配合物 DNA 电化学 嵌插作用 

分 类 号：O641.4[理学—物理化学]