活化沉默信息调节因子1对大鼠严重烧伤早期肾损害的影响  

Effects of activating silent information regulator 1 on early kidney damage in rats with severe burn

作　　者：白晓智[1] 何亭[1] 刘洋[1] 张伟[1] 韩夫[1] 杨晨[1] 蔡维霞[1] 贾艳慧[1] 石继红[1] 韩军涛[1] 苏琳琳[1] 胡大海[1] Bai Xiaozhi He Ting Liu Yang Zhang Wei HaM Fu Yang Chen Cai Weixia Jia Yanhui Shi Jihong HaM Juntao Su Linlin Hu Dahai(Burn Center of PLA, Department of Burns and Cutaneous Surgery, Xifing Hospital, the Fourth Military Medical University, Xi＇an 710032, China)

机构地区：[1]第四军医大学西京医院全军烧伤中心,烧伤与皮肤外科,西安710032

出　　处：《中华烧伤杂志》2017年第6期344-348,共5页Chinese Journal of Burns

基　　金：国家自然科学基金重点项目（81530064）;国家自然科学基金青年科学基金（81501663、81501666）

摘　　要：目的探讨行沉默信息调节因子1（SIRT1）活化对大鼠严重烧伤早期肾损害的影响。方法取30只雄性SD大鼠，按照随机数字表法分为假伤组、单纯烧伤组、SIRT1激动剂组，每组10只。单纯烧伤组和SIRT1激动剂组大鼠背部造成30％TBSAⅢ度烫伤（下称烧伤），伤后即刻分别腹腔注射生理盐水、终质量浓度为1mg／mL白藜芦醇溶液50mL／kg；假伤组大鼠致假伤，伤后即刻腹腔注射生理盐水50mL／kg。伤后24h收集3组大鼠肾脏组织及腹主动脉血，HE染色观察。肾脏组织形态，ELISA法检测血清肌酐、尿素氮含量，蛋白质印迹法检测肾脏组织中SIRT1、Bax、Bcl-2蛋白表达，实时荧光定量RT-PCR法检测肾脏组织中TNF-α、IL-1β、IL-10的mRNA表达。对数据行单因素方差分析、LSD-t检验。结果（1）假伤组大鼠肾小管、肾小球结构完整；单纯烧伤组大鼠肾小管内管型形成，小管结构不清，肾小球固缩；SIRT1激动剂组大鼠肾小管结构较为完整，肾小球固缩程度较单纯烧伤组轻且固缩的肾小球数量较少。（2）单纯烧伤组大鼠血清肌酐、尿素氮含量分别为（67±14）μmol／L、（22．0±4．4）mmol／L，显著高于假伤组的（28±7）μmol／L、（5．5±1．2）mmol／L（t值分别为6．07、11．53，P值均小于0．01）。SIRT1激动剂组大鼠血清肌酐、尿素氮含量分别为（39±9）μmol／L、（14．1±1．7）mmol／L，显著低于单纯烧伤组（t值分别为4．09、4．17，P值均小于0．01）。（3）单纯烧伤组大鼠肾脏组织中SIRT1、Bcl-2蛋白表达量显著低于假伤组（t值分别为16．32、19．58，P值均小于0．01），Bax蛋白表达量显著高于假伤组（t=5．98，P〈0．01）。SIRT1激动剂组大鼠肾脏组织中SIRT1、Bcl-2蛋白表达量显著高于单纯烧伤组（t值分别为6．94、5．37，P值均小于0．01），Bax蛋白表达量显著低于单纯烧伤组（t=3．44，P〈0．01）。（4）�Objective To investigate the effects of activating silent information regulator 1 （ SIRT1 ） on the early kidney damage in rats with severe burn. Methods Thirty healthy male SD rats were divided into sham injury group （SI） , pure burn group （PB） , and SIRT1 activator group （SA） according to the ran- dora number table, with 10 rats in each group. Rats in groups PB and SA were inflicted with 30% total body surface area full-thickness scald （hereinafter referred to as burn） on the back. Immediately after injury, rats in group PB were intraperitoneally injected with normal saline in the dosage of 50 mL/kg, and those in group SA with 1 mg/mL （final mass concentration） resveratrol in the dosage of 50 mL/kg. Rats in group SI were sham injured and intraperitoneally injected with normal saline in the dosage of 50 mL/kg immediately after injury. Kidney tissue and abdominal aorta blood of rats in the three groups were collected at 24 hours after injury. The morphology of kidney tissue was observed after HE staining. The serum content of creatinine and urea nitrogen was determined with enzyme-linked immunosorbent assay. Protein expressions of SIRT1, Bax, and Bcl-2 in kidney tissue were determined with Western blotting, mRNA expressions of tumor necrosis fac- tor-alpha （TNF-α , interleukin-1 beta （IL-1β） , and IL-10 in kidney tissue were determined with real-time fluorescent quantitative reverse transcription polymerase chain reaction. Data were processed with one-way a- nalysis of variance and LSD- t test. Results （ 1 ） In rats of group SI, structures of kidney tubules and glo- meruli were intact. In rats of group PB, structures of kidney tubules were not clear with casts in them, and glomeruli showed pyknosis. In rats of group SA, structures of kidney tubules were relatively intact, and the pyknosis of glomeruli were slighter as compared with that of group PB with fewer glomeruli showing pyknosis. （2） The serum content of creatinine and urea nitrogen in rats of group PB was （67 ± 14

关 键 词：烧伤 肾 细胞凋亡 沉默信息调节因子1 炎症反应 

分 类 号：R644[医药卫生—外科学]