高乌甲素对重度烧伤大鼠疼痛和炎症反应的作用及其机制  

Effects of lappaconitine on pain and inflammatory response of severely burned rats and the mechanism

作　　者：杨成兰[1] 魏在荣[1] 张天华[1] 曾雪琴[1] 吴必华[1] Yang Chenglan Wei Zairong Zhang Tianhua Zeng Xueqin Wu Bihua(Department of Burns and Plastic Surgery, the Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Chin)

机构地区：[1]贵州遵义医学院附属医院烧伤整形外科,563003

出　　处：《中华烧伤杂志》2017年第6期374-380,共7页Chinese Journal of Burns

基　　金：贵州省科教青年英才培养工程项目（黔省专合字[2012]191号）;汇川区科技计划项目

摘　　要：目的探讨高乌甲素（LA）对重度烧伤大鼠疼痛和炎症反应的作用及其机制。方法取40只SD大鼠按随机数字表法（下同）分为健康＋生理盐水组、假伤＋生理盐水组、单纯烧伤组、烧伤＋LA组、健康＋LA组，每组8只。单纯烧伤组、烧伤＋LA组大鼠背部及右后肢造成约32％TBSA深Ⅱ度烫伤（以下称烧伤），假伤＋生理盐水组大鼠致假伤。烧伤＋LA组大鼠于伤前2．0h及伤后即刻与伤后24．0、48．0、72．0h分别腹腔注射1g／LLA溶液4mL／kg，健康＋LA组大鼠于同前各时相点腹腔注射相同剂量的LA溶液，健康＋生理盐水组、假伤＋生理盐水组大鼠于同前各时相点腹腔注射生理盐水4mL／kg。于伤前1．5h及伤后12．5、24．5、36．5、48．5、72．5h，观察致伤大鼠疼痛行为并对大鼠行机械缩足反射阈值（PWMT）检测；未致伤2组大鼠于同前各时相点行相同观测。另取32只SD大鼠分为生理盐水组、三硝基苯基（TNP）-ATP组、米诺环素组、磷酸吡哆醛（PPADS）组，每组8只，均同前致烧伤。伤后48．0h，生理盐水组大鼠鞘内注入10μL生理盐水，TNP—ATP组大鼠鞘内注入30nmol／μLTNP-ATP10μL，米诺环素组大鼠鞘内注入5g／L米诺环素10uL，PPADS组大鼠鞘内注入10nmol／μA。PPADS10μL。注射前后0．5h，测量大鼠PWMT。伤后72．5h，取假伤＋生理盐水组、单纯烧伤组、烧伤＋LA组大鼠脊髓背角组织行免疫荧光染色观察P2X。受体和OX42受体共表达情况，行免疫组织化学染色观察P2X。受体表达并计数阳性细胞；取大鼠静脉血采用ELISA法检测血清TNF—α、IL-1β含量。未致伤2组大鼠于同前时相点行相同观测。对数据行单因素方差分析、重复测量方差分析、SNK检验、配对t检验和Bonferroni校正。结果（1）健康＋生理盐水组、假伤＋生理盐水组、健康＋LA组大鼠各时相点未出现活动异常现象。至伤后72．5h，单纯烧�Objective To explore the effects of lappaconitine （LA） on pain and inflammatory re- sponse of severely burned rats and the mechanism. Methods Forty SD rats were divided into healthy ＋normal saline group, sham injury ＋normal saline group, pure burn group, burn ＋ LA group, and healthy ＋LA group according to the random number table （the same dividing method below） , with 8 rats in each group. Rats in pure burn and burn ＋LA groups were inflicted with about 32% total body surface area deep partial-thickness scald （hereinafter referred to as burn） on the back and right hind. Rats in sham injury ＋normal saline group were sham injured. Rats in burn ＋LA group were intraperitoneally injected with 1 g/L LA solution in the dosage of 4 mL/kg at 2.0 h before injury and post injury hour （PIH） 0 （immediately） , 24.0, 48.0, and 72.0. Rats in healthy ＋LA group were intraperitoneally injected with LA solution in the same dose at the same time points as above, and rats in healthy ＋normal saline and sham injury ＋normal saline groups were intraperitoneally injected with normal saline in the dose of 4 mL/kg at the same time points as above. At 1.5 h before injury and PIH 12.5, 24.5, 36.5, 48.5, and 72.5, the paw withdrawal mechanical threshold （PWMT） of injured rats was detected, and their pain behaviors were observed. The same observation and detection were conducted in rats without injury in the two groups at the same time points as above. Another 32 SD rats were divided into normal saline group, trinitrophenyl （TNP）-ATP group, minocyline group, pyridoxal-phosphate-6-azophenyl-2＇, 4＇-disulfonic acid （PPADS） group, with 8 rats in each group, and all the rats were inflicted with the same burn injury as above. At PIH 48.0, rats in normal saline group were intrathecally injected with 10 μL normal saline; rats in TNP-ATP group were intrathecally injec- ted with 10 μL TNP-ATP in the concentration of 30 nmol/μL; rats in minocyline group were intrathecally in- jected with 10 μL mino

关 键 词：烧伤 疼痛 受体 嘌呤能P2X4 高乌甲素 

分 类 号：R644[医药卫生—外科学]