常压室温等离子体(ARTP)诱变技术选育柱状田头菇(茶树菇)优良菌株研究初探  

A preliminary study on the mutation breeding of good strain of Agrocybe cylindracea by atmospheric and room temperature plasma

作　　者：王谦[1] 黄媛媛[1] 宋长林[1] WANG Qian HUANG Yuan-Yuan SONG Chang-Lin(College of Life Sciences of Hebei University, Baoding, Hebei 071002, China)

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《菌物学报》2017年第6期776-783,共8页Mycosystema

基　　金：河北省现代农业产业技术体系食用菌创新团队(冀农科发2014)~~

摘　　要：本研究利用添加荔枝枝屑的筛选培养基,通过常压室温等离子体(ARTP)诱变技术对柱状田头菇(茶树菇)菌丝体进行诱变处理,共获得67株再生菌株,初筛得到12株再生菌株与出发菌株有较明显的拮抗反应,复筛得到7株再生菌株的长速、长势优于出发菌株,并通过酯酶同工酶电泳技术对这7株再生菌株进行菌种的真实性鉴定。结果确定了一株适宜分解荔枝枝屑的优良再生菌株AL20,其与出发菌株的酯酶同工酶谱联合系数为0.60,是产生了遗传距离的新菌株,且其菌丝长速达3.13mm/d,生物学效率达到73.15%,分别较出发菌株提高0.21mm/d、9.03%,因此初步确定AL20是能够高效利用荔枝枝屑生产的新菌株。This study obtained 67 regenerative strains of Agrocybe cylindracea mutated by atmospheric and room temperature plasma （ARTP） mutagenesis, growing in screening mediums containing litchi sawdust. The preliminary 12 regenerative strains had obvious antagonism against the original ones. After rescreening seven regenerative strains with faster growth and better vitality were obtained, and they were detected by using electrophoresis method to confirm superiority of the regenerative strains. A regenerative strain named AL20 was found to be excellent for decomposition of litchi sawdust, and the association coefficient between strain AL20 and the original strain was 0.6, indicating that strain AL20 was genetically different from the original one; besides, the mycelial growth rate of strain AL20 was 3.13mm/d, and biological efficiency up to 73.15%, respectively 0.21mm/d and 9.03% higher than the original strain, It can be preliminarily confirmed that strain AL20 was a mutant with hish-efficient utilization of litchi sawdust.

关 键 词：柱状田头菇(茶树菇) ARTP诱变技术 荔枝枝屑 酯酶同工酶 

分 类 号：S646[农业科学—蔬菜学]