贯叶金丝桃中贯叶金丝桃素提取及含量测定研究  

Study on the Extraction and Determination of Hyperforine Content in Hypericum Perforatum

作　　者：孟阔[1] 左力[1] 沙延淳 许笑微 才凤 

机构地区：[1]辽宁中医药大学附属第一医院,辽宁沈阳110032 [2]辽宁省食品检验检测院,辽宁沈阳110015 [3]沈阳诺亚荣康生物制药有限责任公司,辽宁沈阳110179

出　　处：《辽宁中医药大学学报》2017年第6期57-58,共2页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金项目(2013010341-401)

摘　　要：目的:采用低温超声波提取贯叶金丝桃素,建立贯叶金丝桃中贯叶金丝桃素含量测定方法。方法:用高效液相色谱法测定。色谱柱:十八烷基硅烷键合硅胶柱,流动相:0.1%磷酸—乙腈(10∶90),流速(1.0 mL/min),检测波长210 nm,柱温:25℃。结果:线性范围:1.8930~4.4170μg。稳定性:RSD=0.70%,n=5。精密度:RSD=1.06%,n=6。重复性:RSD=0.87%,n=5。回收率:99.13%,RSD=1.20%,n=9。符合有关规定,其他方法学考查均符合有关规定。结论:此方法专属性强、重复性好,可用于贯叶金丝桃原料及提取物中贯叶金丝桃素含量测定。Objective： Hyperforine was extracted by ultrasonic in low temperature, a method was established for the determination of hyperforine content in hypericum perforatum. Methods ： The analysis was used with a Cis column and a mobile phase of 0.1% phosphoric acid-aeetonitrile （ 10 ： 90 ）by HPLC. The flow rate was 1.0 mL/min, detection wavelength was 210 nm and column temperature was 25℃ . Results： The linear range was from 1.8930 pc g to 4.4170μg . The RSD of stability, precision and repeatability were respectively 0.70% （n=5）, 1.06% （n=6）,0.87% （n=5）. The recovery rate was 99.13%, and RSD was 1.20% （ n=9 ）. The methodology examination was in accorded with the relevant provisions. Conclusion ： This method was specific and good reproducible, which can he used for the the determination of hyperforine content in hypericum perforatum.

关 键 词：贯叶金丝桃素 低温超声提取 含量测定 HPLC 

分 类 号：R284.2[医药卫生—中药学]