miR-16在金黄色葡萄球菌脓毒症中的表达及意义探讨  

Expression level of miR-16 in Staphylococcus aureus sepsis and its clinical significance

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作  者:王家学[1] 陈燕[2] 刘小香[3] 孙爱华[3] 杨珺[1] 吕火烊[4] 周永烈[4] WANG Jia-xue1 CHEN Yan2 LIU Xiao-xiang3 SUN Ai-hua3 YANG Jun1 LYU Huo-yang4 ZHOU Yong-lie4

机构地区:[1]杭州医学院检验医学院,杭州310053 [2]浙江省台州医院特需病区,台州317000 [3]杭州医学院基础医学部,杭州310053 [4]浙江省人民医院临床检验中心,杭州310014

出  处:《中国人兽共患病学报》2017年第5期427-431,共5页Chinese Journal of Zoonoses

基  金:浙江省自然科学基金(LY16H190006);国家自然科学青年基金(31501581);浙江省教育厅一般科研项目(Y201534394);浙江医学高等专科学校科学研究计划项目(2015XZA02)~~

摘  要:目的探讨miR-16表达水平与金黄色葡萄球菌脓毒症严重程度的关联性,进一步探讨其潜在的临床意义。方法收集金黄色葡萄球菌脓毒症血液标本共计32例,脓毒性休克、严重脓毒症和一般脓毒症各8例,不同年龄段的健康对照24例;另外,收集革兰氏阴性菌脓毒症血液标本8例。用Trizol液裂解全血后提取microRNA,采用荧光定量PCR测定miR-16在不同组的表达水平(2-△△Ct法),用SPSS 13.0软件分析各组之间的统计学差异。采用SPSS 13.0软件将miR-16定量值与相应CRP和PCT值进行相关性分析。结果 miR-16表达水平与金黄色葡萄球菌脓毒症严重程度呈明显的负关联,各实验组与对照组相比均有统计学差异(P<0.001),实验组之间也有统计学差异(P<0.01)。miR-16表达水平与CRP和PCT值呈负相关性,相关系数分别为-0.561和-0.769。结论 miR-16表达与金黄色葡萄球菌脓毒症有明显的负关联性,提示其可能作为该菌脓毒症严重程度的标记物。We investigated correlation between the level of miR-16 expression and the severity of Staphylococcus aureus sepsis, and further explored its potentially clinical significance. Blood samples were collected from 32 patients, including each 8 cases of septic shock, severe sepsis and general sepsis, as well as 8 cases of healthy volunteers. Blood samples from 24 cases of healthy subjects with different ages were measured, and additionally 8 cases of blood samples from gram negative bacteria sepsis were also determined in current study as a control. Trizol solution for the whole blood lysis was added into blood samples, and followed by the extraction of microRNA. The expression levels of miR-16 in different groups were measured by fluorescence quantitative PCR, in which 2-Delta Ct method was used. SPSS software (version 13.0) was used to analyze the statistical differences between the groups, and further analyze the correlation between miR-16 value and the corresponding CRP and PCT values. Results showed that the expression level of miR-16 was negatively correlated with the severity of sepsis. There were statistically significant differences in experimental groups when compared with the control (P〈 0.001), and there was also a statistically significant difference between each experimental group (P〈0.01). We found that the expression level of miR-16 was negatively correlated with CRP and PCT, the correlation coefficients were -0.561 and -0.769 respectively, and trend analysis showed that there was a significantly negative correlation. A significantly negative correlation was found between the miR-16 expression level and severity of sepsis, suggesting that miR-16 may serve as a biomarker for the severity of Staphylococcus aureus sepsis.Staphylococcus aureus

关 键 词:miR-16 金黄色葡萄球菌 脓毒症 

分 类 号:R378.1[医药卫生—病原生物学]

 

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