水稻OsRhoGAP1基因的序列特征及表达特性分析  

Sequence Characterization and Expression Analysis of OsRhoGAP1 Gene in Rice

作　　者：闫鑫甜[1] 黄俊骏[1] 楼晨[1] 葛慧雯 梁卫红[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《生物技术通报》2017年第5期94-101,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31171182);河南省高校科技创新团队支持计划(15IRTSTHN020)

摘　　要：Os Rho GAP1是本实验室前期从水稻幼穗c DNA文库中分离获得的功能未知基因。通过生物信息学和现代分子生物学技术,系统探讨了水稻Os Rho GAP1的组织表达特性和不同激素处理下的表达情况。生物信息学分析表明,Os Rho GAP1具有植物Rho GAP蛋白典型的结构特征,即CRIB基序和Rho GAP结构域。q RT-PCR检测结果显示该基因的表达具有时空特异性,幼苗期主要在根和叶中表达,幼穗期主要在幼穗和叶中表达,成熟期则主要在根中表达。同时,经ABA、SA、GA和Me JA处理后,Os Rho GAP1基因表达量有明显上调,经IAA和6-BA处理后,Os Rho GAP1基因表达量出现显著下调,表明该基因的表达受到多种激素调控。A function-unknown gene was isolated from a cDNA library of young spike of rice and designated as OsRhoGAP1. Baaed on the bioinformatics and modern molteeul^r biqtogy, we systematically surveyed its expression patterns at different development stages and under different hormones treatments. Bioinformatics analysis showed that OsRhoGAP! had typical structural feature of plant RhoGAP protein, including CRIB motif and RhoGAP domain, qRT-PCR analysis revealed that OsRhoGAP1 gene presented specifically spatio-temporal characteristics, i.e., relative high expression levels in root and leave at the seedling stage, in young panicle and leave at the panicle stage, and in root at the mature stage. Furthermore, the transcription of OsRhoGAP1 was strongly induced by ABA, SA, GA, and MeJA, but suppressed by IAA and 6-BA. It was speculated that the expression of OsRhoGAP1 was regulated by hormones.

关 键 词：水稻 OsRhoGAP1基因 序列分析 表达分析 植物激素 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]