多管复合负压吸引装置对静脉淤血皮瓣治疗的研究  

Research of multi-tube composite negative pressure suction device for the treatment of venous congestion flap

作　　者：贾阳[1] 曹莫[1] 

机构地区：[1]河北医科大学第二医院,石家庄050000

出　　处：《中华整形外科杂志》2017年第3期200-206,共7页Chinese Journal of Plastic Surgery

基　　金：河北省科技计划项目（14277749D）

摘　　要：目的比较多管复合负压吸引装置与单管负压吸引装置对静脉淤血皮瓣治疗的效果。方法新西兰白兔15只，每只做2个下腹部皮瓣，共30个，制成静脉淤血皮瓣模型，采用随机数字表法将30个皮瓣分为3组，每组共10个，即多管复合负压吸引装置组（A组）、单管负压吸引装置组（B组）、无负压吸引装置组（c组），分别进行不同负压装置处理观察。每组内10个皮瓣再采用随机数字表法分为取材皮瓣6个，非取材皮瓣4个。取材皮瓣分别于术前、术后4、8、24h和3、7d共6个时相点，采用流式细胞学检测皮瓣组织S期细胞比率和细胞早期凋亡率；非取材皮瓣分别于术后相同各时相点观察皮瓣颜色、肿胀程度、指压反应等大体变化及测定皮瓣温度，并于制成静脉淤血皮瓣模型后第7天，进行各组皮瓣成活面积的测量。结果皮瓣表面温度、皮瓣成活面积、皮瓣组织s期细胞比率3组间比较，差异均有统计学意义（P〈0．05），A组〉B组〉C组；皮瓣组织细胞早期凋亡率（P〈0．05），A组〈B组〈C组。结论使用多管复合负压吸引装置治疗静脉淤血皮瓣，能促进皮瓣组织细胞增殖，减少细胞凋亡，增加静脉淤血皮瓣成活的面积。Objective To compare the multi-tube composite negative pressure suction device with a single tube negative pressure suction device for the treatment of venous congestion flap. Methods 15 New Zealand white rabbits were selected. Two lower abdomen skin flaps were larvested as venous congestion flap models in each rabbit. The 30 flaps were randomly divided into three groups, as group A （multi-tube composite negative pressure suction device, 10 flaps）, group B （the single tube negative pressure suction device, 10 flaps） and group C （no pressure suction device, 10 flaps）. The 10 flaps in each group were again randomly divided into six flaps for histological study and four flaps for observation. The six flaps were harvested at 4,8,24 hours and 3, 7 d after operation for detection of S-phase ratio（ S- phase fraction, SPF） and cell apoptosis by flow cytoinetry. The 4 flaps were observed for flaps color, swelling, finger pressure reaction. The survival rate was measured 7 days after operation. Results The difference between group A and B or between B and C was significant in surface temperature （ group A 〉 B 〉 C, P〈0.05）, flap survival area（ group A〉 B 〉C, P〈0.05）, flap SPF（group A 〉 B 〉C, P〈0.05） and flap early apoptosis（ group A 〈 B 〈 C, P 〈 0.05 ）. Conclusions Compostie negative pressure suction device can treat venous congestion flap, especially multi-tube device. It can promote flap tissue cell proliferation, reduce the apoptosis and increase the flap survival area.

关 键 词：外科皮瓣 静脉淤血 负压吸引 流式细胞术 

分 类 号：R622[医药卫生—整形外科]