玉女煎对气分证兔舌黏膜CK14、E-Cad、HSP70、PCNA、TLR4表达影响  

Influence of Yunu Decoction on Expressions of CK14,E-Cad,HSP70,PCNA,TLR4 in Tongue Mucosa of Rabbit with Qifen Syndrome

作　　者：周韬[1] 张李唯 张军峰[1] 姜淼[1] 马健[1] 詹瑧[1] 佟书娟[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210023

出　　处：《中华中医药学刊》2017年第6期1355-1357,I0001-I0003,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81473593;81473458);江苏省青蓝工程项目;江苏省优势学科建设工程项目(PAPD)

摘　　要：目的:探讨玉女煎对兔舌象变化的分子机制。方法:玉女煎治疗内毒素(Lipopolysaccharide,LPS)所造气分证,观察兔舌象变化,免疫组织化学研究舌黏膜CK14、E-Cad、HSP70、PCNA、TLR4表达变化。结果:气分证兔舌黏膜增厚,CK14、HSP70、PCNA、TLR4表达升高,E-Cad表达降低,玉女煎治疗可以逆转舌黏膜相关分子表达水平,使舌黏膜变薄。结论:气分证兔造模前后及用药前后舌黏膜厚度的变化与CK14、E-Cad、HSP70、PCNA、TLR4表达有密切联系。Objective： To explore the molecular mechanism of Yunu Decoction on changes of tongue picture of rabbit.Methods： Lipopolysaccharide was injected to make rabbit model with Qifen syndrome. After being given Yunu Decoction,changes of tongue picture of rabbit were observed and the expressions of CK14,E-Cad,HSP70,PCNA,TLR4 in the tunica mucosa linguae were assessed through immunohistochemistry. Results： Before treatment,the tongue mucosa of rabbit with Qifen syndrome was thicker; the expressions of CK14,HSP70,PCNA,TLR4 increased and that of E-Cad decreased. While Yunu Decoction could make tongue mucosa thinner by reversing the expression of relevant molecular markers in the tongue mucosa. Conclusion： Either changes of tongue mucosa in rabbit before and after making model,or changes due to medication are closely related to the expressions of CK14,E-Cad,HSP70,PCNA,TLR4.

关 键 词：玉女煎 气分证 舌黏膜 CK14 E-CAD HSP70 PCNA TLR4 

分 类 号：R285.5[医药卫生—中药学]