CD4^+IL-9^+T细胞在实验性动脉粥样硬化中的作用  

Role of CD4^+IL-9^+T cells in experimental atherosclerosis

作　　者：明婷婷 丁绍威 胡超杰[1] 张翠萍[1] 伍权[1] 李庆[1] 

机构地区：[1]安徽医科大学附属省立医院中心实验室,合肥230001

出　　处：《安徽医科大学学报》2017年第6期824-828,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金面上项目(编号:81472018);安徽省自然科学基金面上项目(编号:1408085MH145)

摘　　要：目的探讨CD^4+IL-9^+T细胞(Th9)在载脂蛋白E基因敲除(ApoE^(-/-))小鼠动脉粥样硬化模型中表达变化及意义。方法 8周龄ApoE^(-/-)小鼠高脂饮食饲养,同周龄C57BL/6对照小鼠普通饮食饲养,至32周龄后,测定血清中血脂含量;小鼠心脏主动脉根部石蜡切片,常规HE染色,观察动脉粥样硬化斑块的组织学特点;流式细胞术检测脾脏Th9细胞表达;ELISA法测定血清白介素(IL)-9和氧化低密度脂蛋白(ox-LDL)的浓度。结果 32周龄时,ApoE^(-/-)小鼠血脂水平升高,主动脉根部出现明显脂质斑块,其脾脏Th9细胞表达率、血清IL-9和ox-LDL浓度均明显高于同周龄C57BL/6小鼠(P<0.01)。Th9表达率、IL-9浓度与ox-LDL浓度均呈显著正相关性(P<0.01)。结论 Th9细胞及其分泌的IL-9在动脉粥样硬化发生中发挥重要作用,Th9细胞可成为动脉粥样硬化发病机制研究和干预治疗的重要靶点。Objective To investigate the expression and significance of CD4＋ IL-9 ＋T cells（Th9） in atherosclerosis model of ApoE -/- mice. Methods ApoE -/- mice aged 8 weeks were fed with high fat and their age-matched C57BL/6 control mice were fed with normal diet. The mice were examined at the age of week 8 and week 32. Blood lipid levels in serum were detected. Paraffin sections of aorticroot were used to observe the histological fea- tures of atherosclerotic plaques by HE staining. The frequencies of Th9 cells in spleen were measured by flow cy- tometry. The concentrations of interleukin（IL）-9 and oxidized low density lipoprotein （ox-LDL） in the serum were detected by ELISA assay. Results At the age of week 32, the aortic root of ApoE -/- mice appeared obvious lipid plaques,Th9 cells frequencies and IL-9 concentrations were significantly higher than those of age matched C57BL/ 6 mice（P 〈0.01 ）. Th9 cells frequencies and IL-9 concentration were positively correlated with ox-LDL concentra- tion （P 〈 0.01 ）. Conclusion Th9 cells and IL-9 play an important role in the occurrence of atherosclerosis. Th9 cells may become important target of mechanism research and treatment intervention for atherosclerosis.

关 键 词：TH9细胞 白细胞介素-9 动脉粥样硬化 APOE-/-小鼠 

分 类 号：R392.12[医药卫生—免疫学]