GSTM1和CYP2E1基因多态性与非小细胞肺癌遗传易感性的相关性研究  

Study the Correlation of GSTM1 and CYP2E1 Gene Polymorphism and Genetic Susceptibility to Non-small Cell Lung Cancer in Shenzhen Area

作　　者：刘爱胜 郭龙华 文艳 刘小君 林丽云 房笃智 

机构地区：[1]广东省深圳市龙华新区人民医院检验科,医学检验技术创新研究重点实验室,518109 [2]光明新区人民医院 [3]龙岗区第二人民医院 [4]宝安区人民医院

出　　处：《临床输血与检验》2017年第3期260-265,共6页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：广东省深圳市龙华新区科技局项目(No.507172952023);深圳市龙华新区2015年科技创新基金"重点实验室"项目(No.2015-39)资助

摘　　要：目的探讨细胞色素p4502E1基因(CYP2EI)和谷胱甘肽转硫酶MI(GSTM1)基因多态性与深圳地区非小细胞肺癌遗传易感性的相关性。方法收集2014年2月~2016年10月在深圳各医院就诊并确诊为非小细胞肺癌患者和同期住院的肺良性疾病患者各71例,采用PCR-RFLP和PCR法分别检测CYP2E1基因的RsaⅠ/PstⅠ和GSTM1基因多态性,并分析基因多态性与非小细胞肺癌遗传易感性之间的相关性。结果非小细胞肺癌组和肺良性疾病组患者CYP2E1基因Rsa I/Pst I多态性的三种基因型检出频率差异无统计学意义(χ~2=0.891~1.205,P>0.05);非小细胞肺癌组GSTM1(–)基因型频率为61.79%,显著高于肺良性疾病组的36.62%,两者频率的差异有统计学意义(χ~2=5.019,P<0.05);携带GSTM1(–)基因型的个体患非小细胞肺癌的危险性显著高于GSTM1(+)基因型的个体(OR=2.095,95%CI=1.104~3.173,P=0.032);与携带cl/c2或c2/c2基因型的不吸烟个体比较,携带cl/cl基因型的吸烟者患非小细胞肺癌的风险显著增加(OR=3.415,95%CI=1.092~11.214,P=0.028);携带cl/cl和GSTM1(–)基因型的个体患非小细胞肺癌的风险显著高于携带GSTM1(+)和cl/c2或c2/c2基因型的个体(OR=3.518,95%CI=1.106~l2.812,P=0.045)。在不吸烟人群中,携带GSTM1(–)和cl/cl基因型的人群患非小细胞肺癌的风险显著高于携带GSTM1(+)和cl/c2或c2/c2基因型的人群(OR=2.917,95%CI=1.004~8.316,P=0.043),且携带有GSTM1(–)和cl/c2或c2/c2基因型的人群患非小细胞肺癌的风险同样高于携带GSTM1(+)和cl/c2或c2/c2基因型的人群(OR=14.062,95%CI=1.362~147.256,P=0.029)。结论 GSTM1(–)基因型是深圳地区人群患非小细胞性肺癌的风险因素之一;同时携带CYP2E1的cl/cl和GSTM1(–)基因型可增加吸烟和不吸烟人群患非小细胞肺癌的风险。Objective To explore the correlation between the cytochrome p4502E1 gene transfer （CYP2EI） and glutathione enzyme sulfur MI （GSTM1） gene polymorphism and genetic susceptibility of non-small cell lung cancer in shenzhen area. Methods Samples were collected from February 2014 to October 2016 in shenzhen, every hospital and diagnosed with non-small cell lung cancer patients and at the same time all the 71 patients with lung benign disease in hospital, CYP2E1 gene was detected by PCR method then by the PCR-RFLP in Rsa Ⅰ /Pst Ⅰ , and the correlation between non-small cell lung cancer and gene polymorphism was analysed.Results There was no statistically significant difference between （x2=0.891~1.205, P〉0.05） non-small cell lung cancer and lung benign disease group patients in GSTM1 gene polymorphism, CYP2E1 gene Rsa I/Pst I polymorphisms of the three genotypes detection.; In non-small cell lung cancer group, GSTM1 （-） genotype frequency was 61.79%, significantly higher than 36.62% of lung benign disease group, the difference was statistically significant in genetype frequency between two group （X2=5.019, P〈0.05） ： The risk ofnon-small cell lung cancer is significantly higher in individual carrying GSTM1 （-） genotypes than the GSTM1 （＋） genotype individuals OR 2.095 （95% CI=1.104~3.173, P=0.032） ; with c1/c2 and c2/c2 gene type of nonsmoking individuals, carrying the cl/cl genotypes of smokers significantly increased risk of non-small cell lung cancer （OR= 3.415, 95% CI=1.092~11.214, P=0.028） ; with c1/c1 and GSTM1 （-） genotype individuals had a significantly higher risk of non-small cell lung cancer than individuals with GSTM1 （＋） and cl/c2 and c2 / c2 genotype （ （OR=3.518, 95% CI=3.518~12.812, P=0.045） .In the crowd, smoking is not allowed to carry GSTM1 （-） and c1/c1 genotype had a significantly higher risk of non-small cell lung cancer with GSTM1 （＋） and cl/c2 and c2 / c2 gene type of people （OR=2.917, 95% CI=1.004~8.316, P=0.043） , and w

关 键 词：GSTM1 CYP2E1 基因多态性 深圳 非小细胞肺癌 易感性 

分 类 号：R392.11[医药卫生—免疫学] R734.2[医药卫生—基础医学]