枇杷属植物野生种的茎段愈伤组织诱导植株再生研究  

Study on plant regeneration of callus from the stem of wild loquat

作　　者：刘义存[1,2] 黄天启 林顺权[2] LIU Yicun HUANG Tianqi LIN Shunquan(Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong, China College of Horticulture of South China Agricultural University, Guangzhou 520642, Guangdong, China)

机构地区：[1]仲恺农业工程学院园艺园林学院,广州510225 [2]华南农业大学园艺学院,广州510642

出　　处：《果树学报》2017年第6期762-768,共7页Journal of Fruit Science

基　　金：广东省科技厅科技基础条件建设项目(2014A030304057;2015A030303015);广州市科技创新委资助华南农业大学(亚热带农业生物资源保护与利用)国家重点实验室项目(201504010028)

摘　　要：【目的】建立野生种枇杷茎段愈伤组织诱导植株再生的离体培养技术体系。【方法】以枇杷属植物的2个野生种、1个属间杂交后代为试材,采用茎段为外植体,在不同植物生长调节剂的组合配比培养条件下,开展野生枇杷茎段诱导愈伤组织以及愈伤组织诱导出不定芽的植株再生研究。【结果】最适合野生种枇杷茎段愈伤组织诱导植株再生的培养基配方为:MS+6-BA 1.0 mg·L^(-1)+TDZ 0.1 mg·L^(-1)+NAA 0.1 mg·L^(-1),如栎叶枇杷茎段愈伤组织诱导植株再生,其愈伤率、丛芽率和成苗率均达到100%,不定芽数最多可达38.75个;但对于属间杂交后代的石斑木×台湾枇杷最适合茎段愈伤组织诱导植株再生的培养基配方为:MS+6-BA 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1),其丛芽率和芽苗数量分别为100%和48个,成苗率也为100%;在众多的生长调节剂中,TDZ对野生枇杷的愈伤组织诱导再分化或脱分化是比较敏感的,高浓度的TDZ虽有利于愈伤组织诱导再分化不定芽,但不利于成苗。【结论】研究得出枇杷属植物以及属间杂交后代茎段愈伤诱导植株再生的共性规律和种间的差异性,建立起枇杷属植物野生种离体再生系统,简化植株再生诱导的步骤和缩短植株再生诱导的时间,能在短时间内产生一定数量的组培苗,为枇杷属种质资源研究积累了新的资料。[Objective] The Eriobotrya Lindl. belongs to the genus Maloideae of the Rosaceae subfamily. The loquat originates in the south of China and some countries in Southeast Asia, which are adjacent to the southwest of China and there are more than 30 species or varieties. Breeding of wild loquat and quantitative cultivation test work has just begun. The lack of a system of rapid propagation system, especially the explant material used in tissue culture of wild loquat, is scarce. The stem, which is relative to the wild loquat whole plant, is at the most convenient and rich place. Therefore it is necessary to establish the in vitro culture system for the regeneration of the stem callus of wild loquat. The study on wild loquat stem covers callus induction, bud induction and plantlet formation of adventitious bud growth and regeneration, in order to find suitable methods for induction of stem segment buds, adventitious bud and seedling culture medium combination formula and environmental growth conditions. [ Methods ] This work used the stem of 2 wild species of Eriobotrya Lindl., and 1 interspecific hybrid as materials to study the callus induction and plant regeneration of wild loquats in the different combinations of plant growth regulators and culture conditions. The two wild species are E. prinoides Redh. ＆ Wils and E. bengalensis f. angustifolia Vidal, which were taken from the Yunnan province of China＇ s Shiping county and Kunming city. In addition, a hybrid between the two species of the genus Rhaphiolepis indicaxE, deflexa Nakai is a new species of Loquat Germplasm Resources in the South China Agricultural University. [Results ] The optimum medium for inducing plant regeneration from callus of the stem segment of the wild species of Eriobotrya plants is MS＋ 6-BA 1.0 mg· L^-1 ＋ KT 1.0 mg· L^-1 ＋ NAA 0.1mg· L^-1. For example, in the regeneration of callus from the stems of E. prinoides Redh. ＆Wils, the induction rate, sprouting rate and plantlet rate are all 100%, the maximum number of adventitious b

关 键 词：枇杷属植物 野生种 茎段 离体培养 愈伤组织 植株再生 

分 类 号：S667.3[农业科学—果树学]