益气血法对超排卵大鼠卵巢颗粒细胞凋亡及Bim表达的影响  

Effect of Yiqixue Recipe on Ovary Apoptosis and Expression of Bim in Ovarian Granulosa Cells of Controlled Ovarian Hyperstimulation Rats

作　　者：杨贞[1,2] 郭新宇[1] 邓伟民[3] 张金玉[1] 袁人飞 汤璐璐[3] 

机构地区：[1]广州军区广州总医院生殖医学中心,广州市510010 [2]广州中医药大学,广州市510405 [3]广州军区广州总医院内六科,510010

出　　处：《实用医学杂志》2017年第10期1593-1596,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81403249)

摘　　要：目的:观察益气血法经后增殖方对超排卵大鼠卵巢颗粒细胞凋亡及Bim基因表达的影响,探讨其治疗效果及作用机制。方法:建立超排卵大鼠模型,30只大鼠随机分为空白对照组、阳性对照组、中药低、中、高剂量组,每组6只。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)检测卵巢颗粒细胞凋亡率,实时荧光定量PCR(qPCR)检测Bim基因表达水平。结果:TUNEL:中药高、中剂量组卵巢颗粒细胞凋亡率显著低于阳性对照组(P<0.05),与空白对照组比较无显著差异(P>0.05);qPCR:中药高、中、低剂量组卵巢颗粒细胞Bim基因表达水平显著低于阳性对照组,仍显著高于空白对照组(P<0.05),各剂量组间比较无显著差异(P>0.05)。结论:经后增殖方能抑制Bim基因的表达,抑制卵巢颗粒细胞凋亡,提高超排卵大鼠卵泡质量。Objective To assess the effects of Jinghou Zengzhi Recipe （JHZZR）, a Chinese prescription with the action of tonifying Qi and blood, on the ovary apoptosis and expression of Bim in ovarian granulosa cells （GCs） of controlled ovarian hyperstimulation （COH） rats, and analyze the possible therapeutic mechanism. Methods A model of COH rats were prepaered and 30 female SD rats were randomly divided into 5 groups, including control group, positive control group, low, medium and high concentration group in six rats in each group. The apoptosis index （AI） in ovarian GCs were detected by TUNEL, and the expression of Bim by qPCR. Results The AI of ovarian GCs in high and medium concentration group were obviously lower （P 〈 0.05） than that in the positive control group, and there is no significant differences （P 〉 0.05 ） comparing with control group. The mRNA levels of Bim were lower （P 〈 0.05） in all concentration group than those in positive control group, but still higher（P 〈 0.05 ） than that in control group. And there is no significant differences （P 〉 0.05） during the three concentration groups in Bim mRNA. Conclusion JHZZR can inhibit the ovarian GCs apoptosis of COH rats through decreasing the expression of Bim mRNA, which improve the quality of ovarian follicle.

关 键 词：益气血法 经后增殖方 超排卵 颗粒细胞凋亡 BIM 

分 类 号：R714.8[医药卫生—妇产科学]