石榴皮鞣质改善慢性肾小球肾炎大鼠的尿液代谢组学的通路分析  

Analysis of Urinary Metabonomics Pathway in Rats with Chronic Glomerulonephritis Intervened by Tannins from Pericarpium Granati

作　　者：陈鹏[1] 涂杰[2] 周本宏[1,3] ChenPeng Tu Jie Zhou Benhong(Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, China Centrai Hospital of Wuhan School of Pharmaceutical Sciences, Wuhan University)

机构地区：[1]武汉大学人民医院药剂科,武汉430060 [2]武汉市中心医院 [3]武汉大学药学院

出　　处：《中国药师》2017年第6期969-973,993,共6页China Pharmacist

基　　金：国家自然科学基金项目(编号:31170330)

摘　　要：目的:探讨石榴皮鞣质改善慢性肾小球肾炎大鼠尿液代谢组学的通路。方法:运用KEGG数据库进行信号通路分析,用HMDB数据库对代谢产物分子注释、相关的酶或转运蛋白及其相关性质进行分析,Met PA网络软件对代谢产物路径进行可视化作图。结果:12个差异性代谢产物共参与了16条代谢途径,其中"色氨酸代谢"、"三羧酸循环"、"苯丙氨酸代谢"、"半胱氨酸和甲硫氨酸代谢"的代谢通路影响显著(P<0.05)。结论:石榴皮鞣质干预下的慢性肾小球肾炎大鼠中"色氨酸代谢"、"三羧酸循环"、"苯丙氨酸代谢"、"半胱氨酸和甲硫氨酸代谢"发生变化参与其病理过程。Objective： To analyze the metabolicomics pathway in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati. Methods： Signal pathway analysis was carried out using the KEGG database and molecular metabolite anno- tation was performed using HMDB database. The enzyme or transporter and its related properties were analyzed. The metaholite path visualization was carried out by using MetPA network software. Results ： The analysis of biological metabolism pathway showed that 12 metabolites involved in 16 metabolic pathways. The pathway of tryptophan metabolism, citrate cycle, phenylalanine metabolism, cyste- ine and methionine metabolism showed notable changes （ P 〈 0.05 ）. Conclusion： The changes of phenylalanine metabolism, citrate cycle, tryptophan metabolism, cysteine and methionine metabolism in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati participate the pathological process.

关 键 词：尿液代谢组学 慢性肾小球肾炎 石榴皮鞣质 代谢通路 

分 类 号：R285.5[医药卫生—中药学]