HIV-1急性期感染者肠道归巢α4β7 Th17细胞的变化特点  

Characterization of gut-homing α4β7 Th17 cells in individuals with acute HIV-1 infection

作　　者：吴江[1] 计云霞[2,3] 王蕊[2,3] 陆小凡[2,3] WU Jiang JI Yun-xia WANG Rui LU Xiao-fan(Office for Development of Science and Technology, Center for Disease Control and Prevention of China, Beijing 102206, China)

机构地区：[1]中国疾病预防控制中心科技开发办公室,北京102206 [2]首都医科大学附属北京佑安医院感染中心,北京100069 [3]艾滋病研究北京市重点实验室,北京100069

出　　处：《中国病毒病杂志》2017年第2期141-144,共4页Chinese Journal of Viral Diseases

基　　金：北京市科技计划(D161100000416003);艾滋病研究北京市重点实验室(BZ0089)

摘　　要：目的分析人类免疫缺陷病毒(HIV-1)急性期感染者(acute HIV-1-infected patients,AHIs)肠道归巢α4β7 Th17细胞的变化特点。方法本研究共纳入26例HIV-1急性期感染者、20例HIV-1慢性期感染者和20例健康对照者。采用流式细胞仪检测肠道归巢α4β7Th17细胞的比例和数量,并分析肠道归巢α4β7Th17细胞与CD4^+T细胞、T细胞免疫活化水平之间的相关性。结果与健康对照者比较,HIV-1急性期感染者肠道归巢α4β7Th17细胞的比例(2.61%vs 1.34%,P<0.000 1)和数量(2.29 vs 0.66,P<0.000 1)均显著减少,肠道归巢α4β7 Th17细胞与肠道归巢α4β7 CD4^+T细胞(r=0.42,P=0.02)和CD4^+T细胞(r=0.60,P=0.002)呈显著正相关。HIV-1急性期感染导致T细胞免疫活化水平升高(1.87%vs 15.60%,P<0.000 1),肠道归巢α4β7 Th17细胞与T细胞免疫活化水平呈负相关(r=-0.47,P=0.01)。结论肠道归巢α4β7 Th17细胞的丢失可能在HIV-1致病机制中起作用。Objective To analyze the change of gut-homing α4β7 Th17 cells in individuals with acute HIV-1 in- fection. Methods A total of 26 acute HIV-l-infeeted patients, 20 chronic HIV-1 infected patients, and 20 healthy controls were enrolled in this study. Flow cytometry was performed to analyze the frequency and abso- lute number of gut-homing α4β7 Thl7 cells. Correlations between gut-homing α4β7 Th17 cells and CD4＋ T cell count or the level of T cell activation were also analyzed. Results Comparing to healthy controls, gut-hom- ing α4β7 Th17 cells were significantly decreased in acute HIV-1-infected patients in both frequency and abso- lute number （2.61% vs 1.34%, P〈0. 000 1）. Gut-homing Th17 cells in acutely infected patients were posi- tively correlated with gut-homing CD4＋ T cells （r=0.42, P=0.02） or circulating CD4＋ T ceils （r=0.60, P=0. 002）. HIV-1 acute in{ection also led to elevated level of T cell immune activation （P〈0. 000 1）. Gut-homing Thl7 cells during acute infection stage had a negative correlation with the level of T cell immune activation （r=-0.47, P=0.01）. Conclusions Gut-homing α4β7 Th17 cells may contribute to the HIV-1 pathogenesis.

关 键 词：人类免疫缺陷病毒 急性期感染 肠道归巢α4β7 TH17细胞 

分 类 号：R512.91[医药卫生—内科学]