病毒分离培养新技术及其应用  

Development and application of novel techniques for viral isolation and culture

作　　者：吴冰洁[1] 陆柔剑[2] 谭文杰[1,2] WU Bing-jie LU Rou-jian TAN Wen-jie(Institute of Medical Virology, Wenzhou Medical University, Whenzhou , Zhejiang 325035, Chin)

机构地区：[1]温州医科大学医学病毒学研究所,浙江温州325035 [2]中国疾病预防控制中心病毒病所,北京100052

出　　处：《中国病毒病杂志》2017年第2期154-158,共5页Chinese Journal of Viral Diseases

基　　金：国家重点研发计划项目(2016YFD0500301);国家自然科学基金(81100062)

摘　　要：一些新发病毒和难培养病毒,如人冠状病毒、人诺如病毒、肝炎病毒等,由于缺乏有效培养体系,使这些病毒的疫苗研发、病理机制等深入研究受限。本文总结了传统病毒培养分离技术的发展及其应用,重点介绍了近年来发展的一些新兴技术,如离心培养、混合细胞培养、转基因培养、器官型上皮细胞"木筏式"培养、基质培养模型、旋转壁式生物反应器培养模型、干细胞来源的细胞培养。这些新技术提高了病毒分离培养效率及灵敏度,促进新病毒的发现,为抗病毒药物、疫苗研发奠定物质基础。Studies on viral vaccines and pathological mechanisms were limited by the lack of effective culture systems for viruses such as common respiratory coronavirus, human norovirus, hepatitisand hepatitis virus and so on. In this review, we summarized the recent development on virus isolation and culture by focusing on the viral centrifugation culture, cells co-culture, transgenic cell culture, organotypic epithelial raft cultures, ma trix-based culture, rotating wall vessel bioreactor and stem-derived cell cultures. These new techniques im- proved the efficiency and sensitivity of virus isolation and culture and will help in the discovery of novel viruses and research on antiviral drugs and vaccine development.

关 键 词：病毒分离培养 器官型培养 基质 生物反应器 干细胞 

分 类 号：R373[医药卫生—病原生物学]