Current and future editing reagent delivery systems for plant genome editing  

作　　者：Yidong Ran Zhen Liang Caixia Gao 

机构地区：[1]Genovo Biotechnology Co., Ltd., Tianjin 301700, China [2]State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [3]University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Science China(Life Sciences)》2017年第5期490-505,共16页中国科学（生命科学英文版）

摘　　要：Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and consider the pros and cons of current delivery systems. Finally, we will reflect on possible future directions.Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and consider the pros and cons of current delivery systems. Finally, we will reflect on possible future directions.

关 键 词：genome editing DNA RNA RNP and virus delivery Agrobacterium-mediated transformation biolistic method protoplast transfeetion ttransgene-free genome edited plant 

分 类 号：Q943.2[生物学—植物学]