两种γ计数器测量PTH(1-84和1-34)体外药代的应用研究  

The Application of Two Kinds of Gamma Counter Measurement PTH(1-84 and 1-34) in vitro

作　　者：武红玉[1] 周杏琴[2] 陈永伟[1] 邹美芬[2] 徐栋[2] 钦晓峰[2] 徐希杰[2] WU Hong-yu ZHOU Xin-qing CHEN Yong-wei ZOU Mei-fen XU Dong QIN Xiao-fen XU Xi-jie(Clinical Laboratory）, Jian- gYuan Hospital Affiliated to Jiangsu Institute of Nuclear Medicine. Wuxi 214063 China Key Laboratory of Nuclear Medicine （ Research Department）, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine ,Jiangsu Institute of Nuclear Medicine.)

机构地区：[1]江苏省原子医学研究所附属江原医院,江苏无锡214063 [2]江苏省原子医学研究所(研究部)、卫生部核医学重点实验室、江苏省分子核医学重点实验室

出　　处：《中国辐射卫生》2017年第2期227-229,共3页Chinese Journal of Radiological Health

基　　金：国家自然科学基金项目(81371590)

摘　　要：目的通过两种γ计数器测量注射用重组人甲状旁腺素PTH(1-84)及其代谢产物PTH(1-34)体外药代实验的测量数据,找出进口与国产γ计数器在实际测量中的差异。方法用PE公司Wallac1470 WIZARD与中佳GC-1200γ计数器分别测定同一份PTH样品的cpm,分别建立标准曲线,对仪器的本底计数、相对测量效率、精密度等性能指标进行比较。结果 Wallac1470 WIZARD与GC-1200γ计数器测量PTH(1~84)cpm值的线性关系良好;PE-1470本底值为14±3 cpm、GC-1200本底值为57±33 cpm;PE-1470对^(125)I相对测量效率比GC-1200高出12%左右。结论 PE公司Wallac1470 WIZARD与中佳GC-1200γ计数器操作简单方便;PE-1470的准确、灵敏度略优,两者都适用于放射性体外药代样品的测定。Objective The measurement data of the recombinant human parathyroid hormone PTH （1-84） and its metabolite （1-34） in vitro of the recombinant human parathyroid hormone （PTH） were measured by two kinds of gamma counter,and the difference between the imported and domestic gamma counter was found.Methods Cpm of the some PTH samples were determined with a PTH sample cpm by PE company Wallac1470 WIZARD and Ustc ZonKia GC-1200 γcounter respectively to establish the standard curve of the instrument,the background count,relative measurement efficiency,precision and other performance indicators were compared.Results Wallac1470 WIZARD and GC-1200 gamma counter measuring PTH （1-84） linear relationship between the cpm value is good,the regression equation was：Y（cpm） =25.371x（concentration,R2=0.9982）andY （cpm） =19.181 x （concentration,R2=0.998）;PE-1470 the background value is 14 ＆#177; 3 （cpm）,GC-1200 as the background value 57 ＋33 （cpm）;PE-1470 to 125Ⅰ relative measurement efficiency is about 12% higher than GC-1200;PE-1470 and GC-1200 measurement precision is RSD =0.72% and RSD =1.42% respectively.Conclusion PE Wallac1470 WIZARD and Ustc ZonKia GC-1200 γ counter were simple and convenient to operate,accuracy and sensitivity of PE-1470 were slightly better.Both are applicable to radioactive drug generation samples.

关 键 词：γ计数器 体外药代 测量 

分 类 号：TL81[核科学技术—核技术及应用]