长期种植毛竹林土壤丛枝菌根真菌群落演变趋势  

Effects of Long-term Plantation of Phyllostachys edulis on Evolution of Arbuscular Mycorrihizal Fungus Community in Soil

作　　者：牛利敏[1,2] 秦华[1,2] 徐秋芳[1,2] 邬奇峰[3] 苗惊婧 彭定聪 

机构地区：[1]浙江农林大学环境与资源学院,浙江临安311300 [2]浙江省森林生态系统碳循环与固碳减排重点实验室,浙江临安311300 [3]浙江省临安市农业技术推广中心,浙江临安311300

出　　处：《土壤学报》2017年第3期722-734,共13页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(41671252;41271274);浙江农林大学农林碳汇与生态环境修复研究中心预研基金项目(2013CB03)资助~~

摘　　要：为揭示长期种植毛竹林土壤丛枝菌根(Arbuscular mycorrhizae,AM)真菌群落演变特征,通过磷脂脂肪酸(Phospholipid fatty acid,PLFA)和Illumina Miseq测序平台研究了AM真菌生物量及群落结构的演变趋势。结果表明,长期种植毛竹林土壤养分含量总体呈下降趋势,球囊霉素相关土壤蛋白(Glomalin-related soil protein,GRSP)含量及AM真菌生物量也显著降低(p<0.05),其中易提取态球囊霉素相关土壤蛋白(Easily extractable glomalin-related soil protein,EE-GRSP)含量与有机质、碱解氮、速效钾显著正相关(p<0.05),而AM真菌菌丝生物量(16:1ω5 PLFA)与碱解氮极显著正相关(p<0.01)。长期种植毛竹林显著降低了土壤2~0.25 mm大团聚体比例(p<0.05),且与AM真菌菌丝生物量极显著正相关(p<0.01)。测序结果表明,毛竹林土壤AM真菌以球囊霉属(Glomus)为优势种群,其次为无梗囊霉属(Acaulospora),长期种植毛竹后土壤球囊霉属相对丰度显著增加而无梗囊霉属显著降低(p<0.05)。非度量多维尺度转换排序(Non-metric multidimensional scaling,NMDS)分析显示,对照马尾松林与不同种植年限毛竹林土壤AM真菌群落显著区分(p=0.001),土壤含水量(p=0.005)、碱解氮(p=0.001)、有效磷(p=0.014)对AM真菌群落结构变异具有重要贡献。长期种植毛竹显著降低了AM真菌生物量、球囊霉素相关土壤蛋白含量以及2~0.25 mm大团聚体比例,并改变了AM真菌群落结构,不利于土壤碳固存和维持生态系统稳定。【Objective】Arbuscularmycorrhizal （AM） fungi are ubiquitous in the terrestrial ecosystem and capable of forming mutualistic relationships with most high plants, increase soil carbon sequestration via both direct and indirect pathways, and then play a key role in building up carbon storage in the forest ecosystem. However, little has been reported in the literature about variation of the AM fungus community in the soilunder long-term plantation of moso bamboo （Phyllostachysedulis）. Hence, the primary concern of this study is to explore variation of soil AM fungus in biomass and community structure, and its key affecting factors in the soil under long-term planted moso bamboo forest.【Method】As the moso bamboo forests in this area were mostly converted from masson pine forests one by one in the past years, plots of moso bamboo stands different in plantation age （i.e. 5 a, 9 a, 15 a and 18 a,）for comparison with the plots of natural masson pine forest. Each plot （10 × 10 m） had three replicates and all the plots were laid out at least 10 m apart.Five sampling points were set randomly in each plot, and samples of topsoil （0~20 cm）were collected from the five sampling sites in each plot and thoroughly mixed up to make a single composite sample for each plot.The soil samples were analyzed forbiomass and community structure of AM fungi using the phospholipid fatty acid （PLFA） method and the high-throughput sequencing based on the IlluminaMiseqplatform method, respectively.Glomalin-related soil protein （GRSP） was extracted with citrate solution and measured by spectrophotometer using boving serum albumin as standard. Soil water-stable aggregates were analyzed using the wet-sieving method.【Result】Results show that soil nutrients decreased in content with the moso bamboo plantation increasing in age. Moreover, the 18 year old plots were significantly lower than the 5 plots in both GRSP content and AM fungal biomass. Pearson correlation indicated that the content of easily extracted GR

关 键 词：丛枝菌根真菌 毛竹林 群落结构 磷脂脂肪酸 

分 类 号：S154[农业科学—土壤学]