miR-107对人脑微血管内皮细胞增殖、迁移和血管形成能力的影响  

The effect of miR-107 on proliferation,migration,and tube formation ability of human cerebral microvascular endothelial cells

作　　者：刘文静[1] 薛一雪[2] 王萍[2] 商秀丽[3] LIU Wen-jing XUE Yi-xue Wang Ping SHANG Xiu-li(Department of Geriatrics of the First Affiliated Hospital Department of Neurobiology of College of Basic Medicine Department of Neurology of the First Affiliated Hospital, China Medical University, Shenyang 110001, China)

机构地区：[1]中国医科大学附属第一医院干诊神经内科,沈阳110001 [2]中国医科大学基础医学院神经生物学教研室,沈阳110122 [3]中国医科大学附属第一医院神经内科,沈阳110001

出　　处：《解剖科学进展》2017年第3期284-287,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81100243;81502181);辽宁省科技厅科学技术计划项目(2011225020);辽宁省教育厅基金(L2013296)

摘　　要：目的探讨miR-107对人脑微血管内皮细胞增殖、迁移和血管形成能力的影响。方法miR-107化学模拟物agomir转染人脑微血管内皮细胞,CCK-8细胞活性分析实验和Transwell法分别检测内皮细胞增殖和迁移能力变化,体外血管生成实验检测内皮细胞成管能力的改变。结果内皮细胞转染miR-107化学模拟物agomir显著上调miR-107的表达。与阴性对照组(agomiR-NC组)相比,miR-107过表达组(agomiR-107)内皮细胞增殖和迁移显著减少,体外成管能力显著降低(P<0.05)。结论miR-107能够抑制脑微血管内皮细胞增殖、迁移并降低其体外成管能力。Objective To investigate the effect of miR-107 on proliferation, migration and tube formation ability of human cerebral mierovaseular endothelial ceils （hCMEC/D3）. Methods The miR-107 agonist（agomiR-107） was transiently transfeeted into endothelial eell（EC）. The effects of miR-107 on the EC proliferation, migration and tube formation ability were checked by CCK-8 cell viability, Transwell migration and Matrigel tube formation assays. Results The expressiort [eve[ of miR-144 was significantly upregulated in agomiR-107 group than in the agomir-NC group. Compared with negative control of agomir（agomir-NC） group, the viability of EC was significantly lower in agomir-NC group（P〈0.05）. The number of migrating cells and the relative tubule length were significantly decreased in agomiR-107 group than in agomir-NC group（P〈0.05）. Conclusion miR-107 suppressed human cerebral mierovascular endothelial cells proliferation, migration and tube formation.

关 键 词：miR-107 脑微血管内皮细胞 增殖 迁移 体外成管 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]